食品工业科技2026，Vol.47Issue(2)：437-447,11.DOI:10.13386/j.issn1002-0306.2025010002
巴戟天提取物对慢性束缚抑郁小鼠肠道菌群的影响
Effects of Morinda officinalis How.on Gut Microbiota in Mice with Chronic Restraint Stress Depression
摘要
Abstract
Objective:To investigate the effects and mechanisms of Morinda officinalis How.on the gut microbiota of chronic restraint stress(CRS)depression model mice.Methods:Eight-week-old male Kunming mice were randomly divided into six groups based on their body weight:control,model,fluoxetine,and M.officinalis How.low-(25 mg/kg/d),medium-(50 mg/kg/d),and high-dose(100 mg/kg/d)groups were established.CRS was used to establish a mouse model of depression.Depression-like behavior was observed using the sucrose preference(SPT)and tail suspension tests(TST).Morphological changes in the liver,kidneys,and testes were observed using hematoxylin and eosin(HE)staining.Serum 5-hydroxytryptamine(5-HT)levels were measured using enzyme-linked immunosorbent assay(ELISA).Finally,the gut microbiota were analyzed using 16S rRNA gene amplicon sequencing.Results:Compared with the control group,the sucrose preference rate of mice in the model group was significantly reduced,tail suspension time was significantly prolonged(P<0.05),and serum 5-HT level was significantly increased(P<0.01).This caused varying degrees of damage in the liver,kidneys,and testicles,as well as gut microbiota imbalance.After the intervention,50 mg/kg/d M.officinalis How.showed good antidepressant effects.Its sucrose preference rate was significantly increased,and tail suspension time was significantly shortened(P<0.05).5-HT levels were significantly reduced(P<0.01),liver,kidney,and testicular damage returned to normal levels.The abundance of Proteobacteria was significantly increased(P<0.05),and the abundance of Oscillibacter,Eubacterium_xylanophilum_group,Kurthia and unidentified bacterium were increased(P<0.05).Conclusion:The 50 mg/kg/d M.officinalis How.regulates gut microbiota imbalance and improves depression-like behavior in CRS mice.关键词
抑郁症/巴戟天/肠道菌群/慢性束缚应激/5羟色胺Key words
depression/Morinda officinalis How./gut microbiota/chronic restraint stress/5-hydroxytryptamine分类
医药卫生引用本文复制引用
DENG Tongxing,HE Wenhua,WANG Minli,ZHANG Yinpo,CHEN Xudong,CHANG Cheng,FAN Wenjuan,JIN Shaoju..巴戟天提取物对慢性束缚抑郁小鼠肠道菌群的影响[J].食品工业科技,2026,47(2):437-447,11.基金项目
河南省科技厅科技攻关项目(242102310088,242102111058) （242102310088,242102111058）
漯河市自然科学和工程技术类青年拔尖人才培育计划(漯人才[2024]1号) （漯人才[2024]1号）
漯河市医学动物实验公共技术创新型科技团队项目(漯科[2023]54号) （漯科[2023]54号）
漯河市科技创新领军人才培育计划(漯人才[2024]1号) （漯人才[2024]1号）
漯河医学高等专科学校科技创新项目(KJCX202404,2021LYZTDXM009,2023ZD04). （KJCX202404,2021LYZTDXM009,2023ZD04）
