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椪柑陈皮总黄酮大孔树脂纯化工艺优化及其体外活性分析

ZHANG Jiaqiong LIU Chenxing CAO Yan XU Xiaoqiang SHU Yuewen ZHANG Jun ZHANG Xuechun XIA Qile

食品工业科技2026，Vol.47Issue(2)：239-248,10.
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食品工业科技2026，Vol.47Issue(2)：239-248,10.DOI:10.13386/j.issn1002-0306.2025010149

椪柑陈皮总黄酮大孔树脂纯化工艺优化及其体外活性分析

Optimization of Macroporous Resin Purification Process and in Vitro Activity Study of Total Flavonoids from Citrus reticulata cv.Ponkan Peel

ZHANG Jiaqiong 1LIU Chenxing 2CAO Yan 2XU Xiaoqiang 3SHU Yuewen 3ZHANG Jun 2ZHANG Xuechun 4XIA Qile2

作者信息

  • 1. Southwest Forestry University,Kunming 650224,China||Key Laboratory of Post-harvest Handling of Fruits,Ministry of Agriculture and Rural Affairs,Key Laboratory of Postharvest Preservation and Processing of Vegetables(Co-construction by Ministry and Province),Ministry of Agriculture and Rural Affairs,Zhejiang Key Laboratory of Intelligent Food Logistic and Processing,Key Laboratory of Postharvest Preservation and Processing of Fruits and Vegetables,China National Light Industry,Food Science Institute,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China
  • 2. Key Laboratory of Post-harvest Handling of Fruits,Ministry of Agriculture and Rural Affairs,Key Laboratory of Postharvest Preservation and Processing of Vegetables(Co-construction by Ministry and Province),Ministry of Agriculture and Rural Affairs,Zhejiang Key Laboratory of Intelligent Food Logistic and Processing,Key Laboratory of Postharvest Preservation and Processing of Fruits and Vegetables,China National Light Industry,Food Science Institute,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China
  • 3. Zhejiang ZaoChun Agricultural Development Limited Company,Quzhou 324000,China
  • 4. Southwest Forestry University,Kunming 650224,China
  • 折叠

摘要

Abstract

To investigate the desirable purification process of ponkan Chenpi flavonoids(PCf)and their in vitro antioxidant and hypoglycemic activities,this study extracted PCf using ultrasound-assisted solvent extraction.Four types of macroporous resins were evaluated for their static adsorption-desorption efficiency toward PCf,and HPD-500 resin was identified as the optimal carrier.Single-factor experiments were subsequently conducted to optimize the purification parameters.The antioxidant activity of PCf(before and after purification)were assessed using DPPH radical scavenging rate,ABTS+radical scavenging rate,and ferric ion reducing capacity.The α-glucosidase inhibitory activity was evaluated.Results demonstrated that HPD-500 resin achieved the highest purification efficiency for PCf under optimized conditions:2 of pH,80%ethanol concentration,sample loading flow rate of 4 mL/min,elution flow rate of 3 mL/min,and a sample-to-eluent volume ratio of 1:1.6.Post-purification,the reducing sugar content of PCf was significantly reduced,while the total flavonoid content increased(132.2±9.62 mg RE/g DW).Purified PCf exhibited stronger antioxidant and hypoglycemic activities,with 93.75%±0.82%of ABTS+radical scavenging rate reaching,77.42%±0.91%of DPPH radical scavenging rate,double ferric ion reducing capacity,and 66.84%±0.67%of α-glucosidase inhibitory rate.In summary,HPD-500 resin is suitable for the preliminary separation and purification of ponkan Chenpi flavonoids,and the purified substance demonstrates significant antioxidant activity and inhibitory effect of α-glucosidase,providing a valuable reference for the further development of ponkan Chenpi-based functional products.

关键词

陈皮黄酮/分离纯化/抗氧化/降血糖

Key words

ponkan flavonoids/separation and purification/antioxidant/hypoglycemic

分类

轻工纺织

引用本文复制引用

ZHANG Jiaqiong,LIU Chenxing,CAO Yan,XU Xiaoqiang,SHU Yuewen,ZHANG Jun,ZHANG Xuechun,XIA Qile..椪柑陈皮总黄酮大孔树脂纯化工艺优化及其体外活性分析[J].食品工业科技,2026,47(2):239-248,10.

基金项目

国家重点研发计划资助(2024YFD2100603) （2024YFD2100603）

国家现代农业产业技术体系(CARS-26-04BY). （CARS-26-04BY）

食品工业科技

1002-0306

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