食品工业科技2026，Vol.47Issue(2)：287-296,10.DOI:10.13386/j.issn1002-0306.2025060277
低共熔溶剂提取黑芝麻黑色素工艺优化及其活性分析
Optimization of the Extraction Process of Black Sesame Melanin by Deep Eutectic Solvents and Analysis of Its Bioactivities
摘要
Abstract
To optimize the extraction process parameters and analyze the functional activity of black sesame melanin,this study employed deep eutectic solvents(DES)as the extraction solvent.The effects of various factors,including DES compositions,extraction times,DES water contents,extraction temperatures,DES molar ratios,and solid-liquid ratios on the yield of black sesame melanin.Based on the single-factor experiments,the extraction process of black sesame melanin was optimized by response surface methodology,and its in vitro antioxidant and antibacterial activities were evaluated for efficacy.The results showed that the optimal extraction process was as follows:choline chloride:lactic acid 1:2,reaction time 4.3 h,water content 39%,molar ratio 1:2,and solid-liquid ratio 1:5.6(g/mL),under which the yield of black sesame melanin was 7.3%.XAD-8 resin was used to purify the black sesame melanin,and the color value increased from 197.5 to 297.25,and the melanin content increased from 18.08 mg/g to 25.49 mg/g after purification.The IC50 values of melanin solution for scavenging DPPH·,ABTS+·,hydroxyl radical and superoxide anion were 244,111.6,53.67 and 44.56 μg/mL,respectively.The melanin solution had certain inhibitory effects on the growth of Escherichia coli,Staphylococcus aureus and Bacillus subtilis,and the maximum inhibition zone diameters were 14.03±0.05,14±0.1 and 20.1±0.11 mm,respectively.This study provides a theoretical reference for the green extraction of black sesame melanin and the development and utilization of its functional products.关键词
黑芝麻/黑色素/低共熔溶剂/抗氧化活性/抑菌活性Key words
black sesame seeds/melanin/deep eutectic solvents/antioxidant activity/antibacterial activity分类
轻工纺织引用本文复制引用
YU Yuexin,LI Huizhen,ZHANG Zhijun,ZHOU Boxiang,ZHANG Zichen..低共熔溶剂提取黑芝麻黑色素工艺优化及其活性分析[J].食品工业科技,2026,47(2):287-296,10.基金项目
山西省重点研发计划项目特优农业技术创新专项(2022ZDYF123) （2022ZDYF123）
山西省科技成果转化引导专项(202204021301046). （202204021301046）
