中国食物与营养2025，Vol.31Issue(12)：66-73,8.
ω-3多不饱和脂肪酸改善DSS诱导的小鼠急性结肠炎
Omega-3 Polyunsaturated Fatty Acids Ameliorates DSS-Induced Acute Colitis in Mice
摘要
Abstract
[Objective]Inflammatory bowel disease(IBD)is an inflammatory disease caused by intestinal specific immune abnormalities of regulation,studies have examined the multiple effects of Omega-3 PUFAs,such as anti-inflammatory and immunomodulatory effects.However,the immunomodulatory effect of ω-3 PUFAs on IBD needs further study.[Method]In this study,Fat-1 transgenic mice producing endogenous ω-3 PUFAs and litters of wild-type C57/BL6 mice were used to induce colitis models by dextran sulfate sodium(DSS).The lisease activity index(DAI)score was performed.Colon length was measured and HE staining was performed.Intestinal permeability was detected by FITC-glucan.mRNA levels of inflammatory mediators IL-1β,TNF-α,NF-κβ and iNOS were detected by RT-PCR.ZO-1 protein was detected by immunofluorescence,and Occludin and Claudin 5 were detected by Western blot.[Result]Compared with the WT DSS group,the DAI score was decreased in the Fat-1 DSS group,and colon length was increased.Intestinal goblet cell number was decreased and mucosal damage was improved.mRNA expression levels of IL-1β,TNF-α,NF-κB and iNOS in colon were decreased.The expressions of ZO-1,occludin and claudin 5 were up-regulated,and the content of FITC in serum was decreased.[Conclusion]Omega-3 PUFAs can improve IBD by protecting the intestinal bar-rier and reducing intestinal inflammatory response.关键词
Fat-1小鼠/ω-3多不饱和脂肪酸/结肠炎/葡聚糖硫酸钠Key words
Fat-1/ω-3 PUFAs/Colitis/DSS引用本文复制引用
NI Ben,MA Congcong,WANG Ziping,RONG Shuang,HUANG Qingde,HUANG Fenghong,XU Jiqu..ω-3多不饱和脂肪酸改善DSS诱导的小鼠急性结肠炎[J].中国食物与营养,2025,31(12):66-73,8.基金项目
国家自然科学基金联合基金项目(项目编号:U21A20274) （项目编号:U21A20274）
中国农业科学院科技创新工程(项目编号:CAAS-ASTIP-2016-OCRI) （项目编号:CAAS-ASTIP-2016-OCRI）
现代农业产业技术体系(项目编号:CARS-14). （项目编号:CARS-14）
