现代食品科技2025，Vol.41Issue(12)：27-37,11.DOI:10.13982/j.mfst.1673-9078.2025.12.1337
构树穗多糖的抗氧化活性及对AAPH诱导的人红细胞氧化损伤保护作用
Antioxidant Activity of Polysaccharide from Broussonetia papyrifera Panicle and Its Protective Effect Against AAPH-induced Oxidative Damage in Human Red Blood Cells
摘要
Abstract
In this study,the antioxidant activity and protective effect against AAPH-induced oxidative damage in human erythrocytes of the polysaccharides from Broussonetia papyrifera panicle were studied.Broussonetia papyrifera panicle was taken as the raw material,single factor experiments were designed for a hot water extraction method and the extraction process of Broussonetia papyrifera panicle crude polysaccharide was optimized by the response surface method.The evaluation indicators included the scavenging rate of ABTS+∙,DPPH∙ and ∙OH,the reducing ability of Fe3+,and the protective effect against the AAPH-induced human red blood cell oxidative damage,to study the in vitro antioxidant activity and hemolysis inhibition of the polysaccharides.The results showed that the optimal conditions were:solid-to-liquid ratio 1:32.33(g/mL),extraction temperature 89.89℃,extraction time 116.53 min,under which the yield of crude polysaccharide was 23.67%.In the antioxidant experiments,the scavenging rates of ABTS+∙,DPPH∙ and ∙OH of the polysaccharide were 76.92%,57.81%and 80.76%,respectively.The absorbance of the polysaccharide corresponding to the reducing ability against Fe3+was 1.66 at a polysaccharide mass concentration of 8 mg/mL and the wavelength of 700 nm.Erythrocyte hemolysis experiments showed that the polysaccharide could effectively inhibit the production of ROS and MDA in the erythrocytes treated by AAPH,reduce the conversion rate of GSH to GSSG,and regulate the enzyme activities of CAT,GSH-PX and SOD to restore normal red blood cell levels.The study confirmed that the polysaccharide from Broussonetia papyrifera panicle possessed good antioxidant capacity and can protect erythrocytes from oxidative damage caused by AAPH.This research aimed to provide a scientific foundation for the study on the bioactive material basis of Broussonetia papyrifera panicle polysaccharides,which is of great significance for the research and development of health food products derived from Broussonetia papyrifera panicle.关键词
构树穗多糖/条件优化/抗氧化活性/红细胞溶血Key words
Broussonetia papyrifera polysaccharide/condition optimization/antioxidant activity/erythrocyte hemolysis引用本文复制引用
陈文博,甄迪辉,杨智皓,姜朋庆,杨乾坤..构树穗多糖的抗氧化活性及对AAPH诱导的人红细胞氧化损伤保护作用[J].现代食品科技,2025,41(12):27-37,11.基金项目
河南省高等学校重点科研项目(24B550018) （24B550018）
河南工业大学小麦和玉米深加工国家工程研究中心开放课题资助课题(NL2023008) （NL2023008）
郑州工程技术学院高层次人才科研启动项目(zggk202102) （zggk202102）
郑州市科技局基础研究及应用基础研究项目(zkz202203) （zkz202203）
郑州工程技术学院"青年英才计划" （）
