食品与发酵工业2026，Vol.52Issue(1)：11-17,7.DOI:10.13995/j.cnki.11-1802/ts.042547
代谢工程改造大肠杆菌低pH环境下高效合成5-羟基戊酸
Metabolic engineering of Escherichia coli for efficient synthesis of 5-hydroxyvaleric acid in a low pH environment
摘要
Abstract
5-Hydroxyvaleric acid(5-HV)is an important five-carbon compound with a wide range of applications in the fields of pharmaceuticals,fragrances,and materials.At present,there are few studies on the biological production of 5-HV,which is mostly dis-covered as an intermediate and suffers from low yield and high production cost.In this study,a 5-HV-producing E.coli strain from our la-boratory was modified to synthesize 5-HV without induction by removing some elements of the lactose manipulator in the plasmid,thus avoiding the use of the costly and toxic inducer IPTG.The 5-HV titer was further improved by overexpressing the transcriptional activator of the rate-limiting enzyme aldehyde reductase,yqhC.To reduce the amount of base used during fermentation,the Hfq-DsrA antacid element was introduced into the chassis cells to improve the acid tolerance of the strain.Finally,in a 10 L fermenter,the pH of the fermentation broth was maintained in an acidic environment,and 47.5 g/L of 5-HV was achieved with a yield of 42％(glucose to 5-HV,mass frac-tion),while the amount of alkali added was reduced by 45％.The methodology and results of this study are instructive for the large-scale bioproduction of 5-HV.关键词
5-羟基戊酸/大肠杆菌/yqhC/抗酸元件/低pHKey words
5-hydroxyvaleric acid/Escherichia coli/yqhC/antacid element/low pH引用本文复制引用
张欣雨,孙浩然,陈赞文,徐雪,林姿辰,李乃强..代谢工程改造大肠杆菌低pH环境下高效合成5-羟基戊酸[J].食品与发酵工业,2026,52(1):11-17,7.基金项目
十三五国家重点研发计划项目(2022YFC2106200) （2022YFC2106200）
