食品与发酵工业2026，Vol.52Issue(1)：18-24,7.DOI:10.13995/j.cnki.11-1802/ts.042384
构建大肠杆菌C4途径强化血红素合成
Enhancing heme biosynthesis in Escherichia coli by constructing C4 pathway
摘要
Abstract
Heme is an iron porphyrin derivative composed of a ferrous ion and protoporphyrin.The demand of heme is increasing in fields such as food and medicine.Microbial fermentation is one of the methods for obtaining heme,but the titer is low.In this study,Esch-erichia coli BL21(DE3)was used as chassis strain and genetically modified to obtain a high-yield heme producing strain through metabolic engineering strategies.Firstly,the native C5 heme biosynthetic pathway in E.coli was blocked and a heterologous C4 pathway was intro-duced.The resulting engineered strain demonstrated superior heme production compared to strains with enhanced native C5 pathway or co-existing C4/C5 pathways.Subsequently,a further increase of heme production was achieved by over expressing the key genes of hemB or hemH involved in the heme synthetic pathway in E.coli.Finally,by optimizing the supplement of the precursors of glycine and ferrous chloride,the heme content in the engineered strain reached 82.39 μmol/L after 24 h of shaking flask fermentation,which was 4.2-fold compared to that of the starting strain.It is a new strategy to construct an engineered E.coli which uses sole heterologous C4 pathway to produce heme with high titer.关键词
血红素/大肠杆菌/C4途径/C5途径/代谢工程Key words
heme/E.coli/C4 pathway/C5 pathway/metabolic engineering引用本文复制引用
王子晨,张鑫,唐蕾..构建大肠杆菌C4途径强化血红素合成[J].食品与发酵工业,2026,52(1):18-24,7.基金项目
111引智计划项目(111-2-06) （111-2-06）
国家轻工技术与工程一流学科自主课题资助项目(LITE 2018-27) （LITE 2018-27）
