食品与发酵工业2026，Vol.52Issue(1)：31-38,8.DOI:10.13995/j.cnki.11-1802/ts.042452
一种功能性奶粉对去卵巢大鼠骨骼健康的影响
Effect of a functional milk powder on bone health in ovariectomized rats
摘要
Abstract
The present study aims to investigate the effects of a functional milk powder supplemented with Davallia trichomanoides Blume on bone health in ovariectomized rats and its potential mechanisms.Thirty SPF-grade female SD rats weighing approximately 300 g were randomly divided into a sham-operated group,an ovariectomized(OVX)control group and an OVX+milk powder group,and were gavaged for 90 d.Calcium metabolism experiments were performed on the last three days of the intervention period.At the end of the inter-vention,the routine indexes of femur,bone mineral density and bone calcium were detected;The bone microstructure of femur and lumbar spine was scanned by Micro-CT,and key proteins expressions of bone metabolism of lumbar spine were analyzed by immunohistochemis-try.In addition,the mRNA expression levels of Wnt3a,β-catenin,RUNX2,and LEF-1 in the signaling pathways related to the mainte-nance of bone homeostasis were detected in the right tibia.Results showed that compared with the sham-operated group,the OVX control rats showed a significant decrease in the bone mineral density of the distal femur(P＜0.01),and the midpoint bone mineral density and bone calcium levels were significantly reduced in the OVX control group(P＜0.05).Compared with the OVX control group,the OVX+milk powder group showed significantly higher bone mineral density and bone calcium levels at the distal and midpoint of the femur,as well as different degrees of improvement in bone microstructure(P＜0.05).Compared with the OVX control group,the OVX+milk pow-der group showed significantly higher levels of VEGF protein expression in the lumbar spine(P＜0.01),and significantly higher levels ofβ-catenin,RUNX2,and LEF-1 mRNA expression in the tibia(P＜0.05 or 0.01).This functional milk powder can promote the absorp-tion and utilization of calcium,increase bone mineral density and bone calcium content,and thus improve the bone health of ovariecto-mized rats,and its mechanism may relate to Wnt/β-catenin signaling pathway.关键词
骨碎补/去卵巢大鼠/功能性奶粉/骨骼健康/Wnt/β-catenin信号通路Key words
Davallia trichomanoides Blume/ovariectomized rats/functional milk powder/bone health/Wnt/β-catenin signaling pathway引用本文复制引用
何骏,许子骐,苟宇睿,黄淑岚,吴文婧,吕晓华,包兴宇,袁亚,李晓蒙..一种功能性奶粉对去卵巢大鼠骨骼健康的影响[J].食品与发酵工业,2026,52(1):31-38,8.基金项目
国家乳业技术创新中心中老年增肌及健骨功能性乳制品开发项目(2022-KYGG-5) （2022-KYGG-5）
实验学科振兴计划项目(2023SY-03) （2023SY-03）
