食品与机械2025，Vol.41Issue(12)：45-50,6.DOI:10.13652/j.spjx.1003.5788.2025.80209
UPLC-MS/MS同时测定石斛中石斛碱及4种联苄类化合物含量
Simultaneous determination of dendrobine and four bibenzyl compounds in dendrobium by UPLC-MS/MS
摘要
Abstract
[Objective]To develop a method for determining the contents of dendrobine,dendrophenol,moscatilin,chrysotoxine,and erianin in dendrobium by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).[Methods]The samples were ultrasonically extracted with 70%methanol(by volume)for 40 min.The extract was filtered through a 0.22 μm organic membrane and then determined by UPLC-MS/MS.Gradient elution was performed using methanol and 0.1%formic acid water as the mobile phase.The determination was carried out in MRM mode with an electrospray ionization(ESI)source in positive ion mode,and quantification was done by the external standard method.[Results]Dendrobine,dendrophenol,chrysotoxine,moscatilin,and erianin show a good linear relationship in the range of 50.0～500.0 ng/mL(R2>0.999).The detection limits of the five substances are 0.15～0.71 μg/kg,and the quantification limits are 0.50～2.37 μg/kg.The average recovery rates of the five substances at three spiked concentrations(50.0,100.0,and 150.0 μg/kg)are in the range of 95.34%～105.00%,with relative standard deviations of 0.80%～1.88%(n=3).Six batches of commercially available dendrobium samples were determined.The contents of dendrobine,dendrophenol,moscatilin,and erianin are in the range of 0.046%～0.318%,0.024%～0.170%,0.001%～0.099%,and 0.013%～0.099%,respectively.Chrysotoxine is detected in three batches,with contents in a range of 0.001%～0.004%.[Conclusion]A method for determining the contents of dendrobine,dendrophenol,moscatilin,chrysotoxine,and erianin in dendrobium is developed by UPLC-MS/MS.This method is accurate,has a short analysis time and high sensitivity,and is suitable for evaluating the quality of dendrobium.关键词
UPLC-MS/MS/石斛/石斛酚/杓唇石斛素/豉槌石斛素/毛兰素/石斛碱Key words
UPLC-MS/MS/dendrobium/dendrophenol/moscatilin/chrysotoxine/erianin/dendrobine引用本文复制引用
李占彬,陆洋,陶光灿,吴阳,马凯..UPLC-MS/MS同时测定石斛中石斛碱及4种联苄类化合物含量[J].食品与机械,2025,41(12):45-50,6.基金项目
贵州省高层次创新型人才(百层次人才)(编号:黔科合平台人才-GCC[2023]004) （百层次人才）
贵州省科研机构创新能力建设项目(编号:黔科合服企[2019]4001) （编号:黔科合服企[2019]4001）
