中国酿造2026，Vol.45Issue(1)：65-71,7.DOI:10.11882/j.issn.0254-5071.2026.01.009
河套和鹰潭地区中高温大曲细菌类群结构和基因功能差异分析
Analysis on the difference of bacterial flora structure and gene function of medium-high-temperature Daqu in Hetao and Yingtan area
摘要
Abstract
Bacterial flora structure of medium-high-temperature Daqu in the Hetao area of Bayannur,Inner Mongolia was analyzed by high-throughput sequencing technology,the comparative analysis of Alpha and Beta diversity was conducted with the bacterial flora of medium-high-temperature Daqu in Yingtan area of Jiangxi,the differential flora was analyzed by the linear discriminant analysis effect size(LEfSe),and the bacterial gene func-tion was annotated.The results showed that the richness and diversity of bacterial flora in Daqu from the Hetao area were extremely significantly low-er than those in the Yingtan area(P＜0.01).Principal coordinates analysis(PCoA)could effectively distinguish medium-high-temperature Daqu from the two regions.The relative abundance of dominant bacterial genera(relative abundance＞1.0％)such as Saccharopolyspora,Staphylococcus and Ther-moactinomyces in the medium-high-temperature Daqu in the Hetao area was extremely significantly higher than that in the Yingtan area(P＜0.01),while the relative abundance of Bacillus,Aquabacterium and Acinetobacter was extremely significantly lower than that in the Yingtan area(P＜0.01).The result of LEfSe showed that the differential flora of medium-high-temperature Daqu from the two regions were Bacillus and Saccharopolyspora(LDA value＞4.0,P＜0.01).The expression level of metabolism,information storage and processing,as well as cellular process and signal transduction gene function in the medium-high-temperature Daqu in the Hetao area was extremely significantly lower than those in the Yingtan area(P＜0.01).In conclusion,there were certain differences in the bacterial groups of medium-high-temperature Daqu between the two regions.关键词
不同地区/中高温大曲/细菌菌群多样性/功能预测/线性判别分析效应大小Key words
different area/medium-high-temperature Daqu/bacterial flora diversity/function prediction/linear discriminant analysis effect size分类
轻工纺织引用本文复制引用
张青,侯强川,李擎,张敏,任国军,王耀,郭壮..河套和鹰潭地区中高温大曲细菌类群结构和基因功能差异分析[J].中国酿造,2026,45(1):65-71,7.基金项目
襄阳市重大科技计划项目(2020AAT002153) （2020AAT002153）
湖北文理学院教师科研能力培育基金"科技创新团队"(2020kypytd009) （2020kypytd009）
