中国酿造2026，Vol.45Issue(1)：72-78,7.DOI:10.11882/j.issn.0254-5071.2026.01.010
乳扇中真菌菌群多样性分析及酵母菌的分离鉴定
Analysis of fungal community diversity and isolation and identification of yeast in Rushan
摘要
Abstract
To explore the diversity of fungal communities and the differences in culturable yeasts from different sources of Rushan,the fungal commu-nity diversity of 5 Rushan samples collected from Dali Bai Autonomous Prefecture,Yunnan Province was analyzed by high-throughput sequencing technology,and the yeasts in the Rushan were isolated and identified,combined with traditional microbial culture separation and molecular biology techniques.The results showed that there were significant differences in the richness and diversity of fungal communities among the 5 Rushan samples(P＜0.05).The dominant fungal genera(average relative abundance＞1％)in the Rushan samples were Candida,Aspergillus,Debaryomyces,Dekkera,Rhodotorula,Clavispora and Kluyveromyces.A total of 23 yeast strains were isolated and identified from 5 Rushan samples and classified into 6 genera and 8 species,including 7 strains of Candida zeylanoides and 6 strains of Torulaspora delbrueckii,1 strain of Kluyveromyces lactis,1 strain of Candida parapsilosis,1 strain of Pichia cactophila,2 strains of Kluyveromyces marxianus,2 strains of Rhodotorula mucilaginosa,and 3 strains of Clavispora lusitaniae.In conclusion,there were differences in the diversity of the fungal communities and the composition of culturable yeasts of Rushan from different sources.关键词
乳扇/高通量测序/真菌菌群多样性/酵母菌/分离/鉴定Key words
Rushan/high-throughput sequencing/fungal community diversity/yeast/isolation/identification分类
轻工纺织引用本文复制引用
陈玮玮,何宗甫,金娜,李盈增,黄勇桦,张秋燕,郭继英..乳扇中真菌菌群多样性分析及酵母菌的分离鉴定[J].中国酿造,2026,45(1):72-78,7.基金项目
普洱市2024年第二批自筹经费科技项目(ZCXM20240316) （ZCXM20240316）
滇西应用技术大学普洱茶学院院级项目(2024年) （2024年）
云南省首席技师专项(YNWR-SXJS-2020-068) （YNWR-SXJS-2020-068）
