中外葡萄与葡萄酒Issue(1)：38-45,8.DOI:10.13414/j.cnki.zwpp.2026.01.005
外源H2O2对'无核白'葡萄ROS积累的影响
Effects of Exogenous H2O2 on the Accumulation of ROS in'Thompson Seedless'Grapevine
摘要
Abstract
To investigate the distribution and response mechanisms of exogenous hydrogen peroxide(H2O2)on reactive oxygen species(ROS)accumulation pattern in grapes,tissue culture seedlings and potted seedlings of'Thompson Seedless'were treated with root incubation(0-6 mmol·L-1,7 d)and foliar spraying(0-800 mmol·L-1)H2O2 applications.ROS accumulation was visualized using nitrotetrazolium blue chloride(NBT)and diaminobenzidine(DAB)staining,while activities of catalase(CAT),superoxide dismutase(SOD),and glutathione reductase(GR)were determined.Results demonstrated that H2O2 were significantly accumulated in leaf margins but not around leaf veins when roots were treated with 2 mmol·L-1 H2O2.Leaf SOD activity peaked at the 2 mmol·L-1 treatment.Leaf CAT,GR and SOD activities were all significantly enhanced in 100 mmol·L-1 pot seedling treatment group.These findings indicate that different exogenous H2O2 applications affect ROS accumulation patterns in leaves.This study provided insights and knowledge for grape stress response mechanisms.关键词
H2O2/ROS/'无核白'葡萄/抗氧化酶活性Key words
H2O2/ROS/'Thompson Seedless'grapevine/antioxidant enzyme activity分类
农业科技引用本文复制引用
姜臣哲,鄢国英,刘萌萌,潘学军..外源H2O2对'无核白'葡萄ROS积累的影响[J].中外葡萄与葡萄酒,2026,(1):38-45,8.基金项目
贵州大学博士科研启动经费(2022-059) （2022-059）
贵州大学实验室开放项目(SYSKF025-021) （SYSKF025-021）
