食品工业科技2026，Vol.47Issue(3)：392-402,11.DOI:10.13386/j.issn1002-0306.2024120031
中国弯颈霉发酵液代谢物分析及其抗氧化活性评估
Analysis of Metabolites in Fermentation Broth of Tolypocladium sinense and Evaluation of Their Antioxidant Activity
摘要
Abstract
This study aimed to investigate the major metabolites of Tolypocladium sinense fermentation broth(TSFB)and evaluate its in vitro antioxidant activity,as well as its ability to alleviate hydrogen peroxide(H2O2)-induced oxidative stress in mouse hippocampal neuronal cells(HT22).Ultra-high performance liquid chromatography-mass spectrometry(UHPLC-MS)was employed for untargeted metabolomic analysis of TSFB.An antioxidant evaluation system combined with Western blotting was used to assess the antioxidant effects of TSFB and its influence on the expression levels of superoxide dismutase 1(SOD1),nuclear factor erythroid 2-related factor 2(Nrf-2),superoxide dismutase 2(SOD2),and heme oxygenase-1(HO-1).The results revealed that detected 2745 metabolites from TSFB,primarily composed of lipids and lipid-like molecules,organic acids and derivatives,and organoheterocyclic compounds.The dominant bioactive constituents included sphingosine,citric acid,and phytosphingosine,which exhibited the highest relative peak areas.In vitro antioxidant assays demonstrated that TSFB possessed strong free radical scavenging capacity.In the oxidative stress cell model,the results showed that both low and high doses of TSFB treatment significantly increased cell survival rates(P<0.001).Meanwhile,oxidative stress markers such as MDA content,LDH levels,and relative ROS levels were significantly reduced(P<0.05),while the antioxidant capacity indicator GSH-Px levels were significantly elevated(P<0.05).Additionally,the high-dose TSFB group notably increased SOD levels(P<0.01).Both low-and high-dose TSFB treatment groups significantly upregulated the protein relative expression of Nrf-2,HO-1,and SOD1(P<0.05),and the high-dose group further significantly increased SOD2 protein relative expression(P<0.001).This study systematically analyzed the major metabolites and bioactive components of TSFB,elucidating its antioxidant mechanisms and highlighting its potential therapeutic applications.关键词
中国弯颈霉/发酵液/代谢物/细胞凋亡/抗氧化/氧化应激Key words
Tolypocladium sinense/fermentation broth/metabolites/cell apoptosis/antioxidant/oxidative stress分类
医药卫生引用本文复制引用
边聪,张畅,卢德霜,胡月,李超君,李林..中国弯颈霉发酵液代谢物分析及其抗氧化活性评估[J].食品工业科技,2026,47(3):392-402,11.基金项目
黑龙江省"优秀青年教师基础研究支持计划"项目(YQJH2024282) （YQJH2024282）
齐齐哈尔医学院研究生创新基金项目(QYYCX2023-09). （QYYCX2023-09）
