食品工业科技2026，Vol.47Issue(3)：420-431,中插3-中插5,15.DOI:10.13386/j.issn1002-0306.2025020154
基于肠道菌群和代谢组学研究牡蛎多糖改善小鼠2型糖尿病的作用机制
Mechanism of Oyster Polysaccharides in Improving Type 2 Diabetes in Mice Based on Gut Microbiota and Metabolomics
摘要
Abstract
This study investigated the therapeutic mechanism of oyster polysaccharide(OPS)on type 2 diabetes mellitus(T2DM).A T2DM mouse model was established through high-fat diet combined with intraperitoneal injection of streptozotocin(STZ).The experimental mice were divided into six groups:normal control(NC),model control(MC),low-dose OPS(OPS-L),medium-dose OPS(OPS-M),high-dose OPS(OPS-H),and positive control(metformin,MET).Comprehensive evaluations were conducted,including blood glucose and lipid profiles,hepatic and renal histopathology,16S rDNA-based gut microbiota analysis,and untargeted fecal metabolomics.Compared to the MC group,the OPS-L and OPS-M groups exhibited significant reductions in fasting blood glucose(FBG),fasting serum insulin(FINS),and homeostatic model assessment of insulin resistance(HOMA-IR)(P<0.01,P<0.001),along with increased body weight(P<0.05).Serum levels of triglycerides(TG),total cholesterol(TC),and low-density lipoprotein cholesterol(LDL-C)were reduced to varying degrees,with alleviated hepatic and renal tissue damage.Notably,the OPS-M group demonstrated the most pronounced hypoglycemic effects.OPS treatment modulated gut microbiota composition in T2DM mice,restoring α-and β-diversity indices toward NC group levels.Serum metabolomics revealed alleviated metabolic pathways involving histidine metabolism,alpha-linolenic acid metabolism,and ketone body synthesis/degradation.Correlation analysis identified significant associations between differential bile acid metabolites and specific bacterial taxa,particularly g_Muribaculaceae and g_Rikenellaceae_RC9_gut_group.This study indicates OPS ameliorates T2DM in mice through mechanisms potentially linked to gut microbiota modulation,fecal metabolite regulation,and alterations in serum bile acid profiles.关键词
牡蛎多糖/2型糖尿病/肠道菌群/代谢组学/胆汁酸Key words
oyster polysaccharide/type 2 diabetes mellitus/intestinal microbiota/metabolomics/bile acid分类
医药卫生引用本文复制引用
韩云琰,翟兴月,江华,刘献萍,吴松岩,范焱杰,简雨娴,郭冬洁,李林格,佟长青..基于肠道菌群和代谢组学研究牡蛎多糖改善小鼠2型糖尿病的作用机制[J].食品工业科技,2026,47(3):420-431,中插3-中插5,15.基金项目
辽宁省自然基金面上项目(2023-MS-271). （2023-MS-271）
