食品工业科技2026，Vol.47Issue(3)：22-31,10.DOI:10.13386/j.issn1002-0306.2024100152
基于UPLC-Q-Exactive-MS/MS及网络药理学探讨喷雾干燥桑叶酚类物质抗衰老潜在作用机制
Exploring the Potential Mechanism of Anti-aging Effects of Spray-dried Mulberry Leaf Phenolics Based on UPLC-Q-Exactive-MS/MS and Network Pharmacology
摘要
Abstract
Objective:Ultra-high performance liquid chromatography-quadrupole-electrostatic orbitrap mass spectrometry(UPLC-Q-Exactive-MS/MS)and network pharmacology methods were used to study the metabolic absorption and potential biological activity of spray-dried mulberry leaves.Methods:UPLC-Q-Exactive-MS/MS was used to detect the phenolic components of spray-dried mulberry leaves in simulated in vitro digestion,intestinal microbial fermentation and in vivo metabolic processes.Based on the detected phenolic components,the corresponding material targets were found through the database analysis platform Swiss Target and Pharmmapper,and the aging targets were obtained by searching the HAGR and Genecards databases.The PPI(Protein-Protein Interaction)network was constructed using String11.5 database and Cytoscape 3.9.1 software.DAVID 6.8 was used for gene ontology(GO)annotation and KEGG(Kyoto Encyclopedia of Genes and Genomes Analysis)pathway enrichment analysis to analyze core targets and action pathways,and then the"components-targets-pathway"network was constructed.Results:The phenolic compounds of spray-dried mulberry leaves mainly underwent hydrolysis or deglycosidation during the gastrointestinal digestion stage.After being absorbed,these substances mainly underwent phase Ⅰ and Ⅱ reactions such as reduction,hydroxylation,glucuronidation and sulfation in the body.The prototype compounds and metabolites of phenolic compounds in spray-dried mulberry leaves co-existed in the blood circulation.Through the network pharmacology method,476 intersection targets of phenolic substances and aging were screened out.After constructing PPI,47 core targets were screened out.The top ten targets were GAPDH(glyceraldehyde-3-phosphate dehydrogenase),ALB(albumin),AKT1(protein kinase B1),INS(insulin),ACTβ(β-actin),TP53(tumor protein p53),TNF(tumor necrosis factor),IL6(interleukin 6),EGFR(epidermal growth factor receptor),IL1β(interleukin 1β).KEGG results showed that phenolic compounds of spray-dried mulberry leaves might play an anti-aging role by regulating Wnt and TGF-β signaling pathways.Conclusion:The phenolic compounds of spray-dried mulberry leaves may play an anti-aging role through multi-component,multi-target and multi-pathway.关键词
桑叶/高效液相色谱质谱联用/酚类物质/抗衰老/网络药理学Key words
mulberry leaves/UPLC-MS/phenolic compounds/anti-aging/network pharmacology分类
医药卫生引用本文复制引用
檀朝立,章旭,谢雨晴,盛晶晶,潘瑶,俞燕芳,陈芳..基于UPLC-Q-Exactive-MS/MS及网络药理学探讨喷雾干燥桑叶酚类物质抗衰老潜在作用机制[J].食品工业科技,2026,47(3):22-31,10.基金项目
江西省研究生创新专项资金项目(YC2021-S175). （YC2021-S175）
