食品科学2026，Vol.47Issue(2)：300-306,7.DOI:10.7506/spkx1002-6630-20250818-132
线性回文脱氧核酶用于茶叶铅离子快速检测
Linear Palindromic DNAzyme for Rapid Detection of Lead Ions in Tea
摘要
Abstract
Objective:To establish a rapid method based on a linear palindromic DNAzyme for detecting lead ions as a heavy metal contaminant in tea and to meet the demand for rapid on-site detection of heavy metals in real samples.Methods:A lead-specific DNAzyme was designed and constructed,with palindromic sequences introduced at both ends of the enzyme strand to induce linear assembly,thereby enhancing structural stability and recognition efficiency.The enzyme and substrate strands were respectively labeled with BHQ1 and FAM to construct a fluorescence-based sensing system.Key parameters,including the concentrations of enzyme and substrate strands as well as reaction time,were optimized to determine the optimal experimental conditions.Results:Under the optimal conditions,the method exhibited excellent selectivity and sensitivity toward lead ions,with a limit of detection(LOD)as low as 0.012 nmol/L.The recoveries from spiked tea infusion samples ranged from 96.05%to 100.41%,indicating that the detection system has good applicability and reliability in complex sample matrices.Conclusion:The linear palindromic DNAzyme-based method is characterized by simple operation,rapidity,high selectivity,and high sensitivity.It can be effectively applied for the rapid detection of lead ions in tea and other real samples,providing a practical and efficient strategy for food safety monitoring.关键词
回文结构/脱氧核酶/铅离子/荧光检测Key words
palindromic structure/DNAzyme/lead ions/fluorescence-based detection分类
轻工纺织引用本文复制引用
苏超,彭育勃,姚柏均,陈赵然,陈伟..线性回文脱氧核酶用于茶叶铅离子快速检测[J].食品科学,2026,47(2):300-306,7.基金项目
安徽省优秀青年骨干教师国内访问研修项目(gxgnfx2020143) （gxgnfx2020143）
安徽省质量工程项目食品营养与健康教学创新团队项目(2024cxtd203) （2024cxtd203）
芜湖职业技术大学食品营养与健康创新团队项目(2023jxtd06) （2023jxtd06）
