食品工业科技2026，Vol.47Issue(3)：163-171,9.DOI:10.13386/j.issn1002-0306.2025010085
基于转录组测序的新疆伊犁野生阿魏菇子实体发育及多糖合成相关基因的挖掘
Mining of Genes Involved in Fruiting Body Development and Polysaccharide Synthesis of Wild Pleurotus ferulae in Yili Xinjiang Based on Transcriptome Sequencing
摘要
Abstract
This study investigated the gene expression differences between the fruiting body and mycelium stages of Pleurotus ferulae through transcriptome sequencing and bioinformatics analysis,focusing on the molecular mechanisms underlying fruiting body development and polysaccharide biosynthesis.The results revealed that 6458 genes were co-expressed in both fruiting body and mycelium,while the mycelium exhibited a significantly higher number of unique genes(12218)compared to the fruiting body(1022),suggesting that fruiting body-specific genes predominantly drive morphogenesis and the biosynthesis of bioactive compounds.Further comparative analysis using the mycelium transcriptome as a control identified 5339 significantly differentially expressed genes(DEGs)(60 upregulated,5279 downregulated)in the fruiting body transcriptome.These DEGs were enriched in pathways related to carbohydrate metabolism,energy metabolism(including oxidative phosphorylation and the TCA cycle),and genetic information processing,revealing a substantial activation of metabolic activity during fruiting body development.Furthermore,9 polysaccharide biosynthesis-related pathways were pinpointed from 73 KEGG pathways,and 23 key enzyme genes were identified.These genes were primarily associated with pyruvate metabolism,starch and sucrose metabolism,glycolysis/gluconeogenesis,and amino sugar/nucleotide sugar metabolism,thereby delineating core metabolic nodes in polysaccharide biosynthesis.This study pioneered the molecular regulatory framework governing the development and polysaccharide biosynthesis in Pleurotus ferulae.It provided critical theoretical foundations and genetic resources for elucidating the synthesis mechanisms of bioactive polysaccharides,enhancing cultivation efficiency and processing adaptability for food applications,and developing molecular marker-driven quality improvement techniques.These findings laid a molecular foundation for functional food development(e.g.,polysaccharide-based thickeners and nutritional enhancers)and the targeted breeding of high-value-added mushroom products tailored to the food industry.关键词
阿魏菇/转录组/差异分析/发育/多糖合成通路Key words
Pleurotus ferulae/transcriptome/differential analysis/development/polysaccharide synthesis pathway分类
生物科学引用本文复制引用
木古丽·木哈西,玛依拉·吐尔地别克,吾尔恩·阿合别尔迪,王广权,庞克坚..基于转录组测序的新疆伊犁野生阿魏菇子实体发育及多糖合成相关基因的挖掘[J].食品工业科技,2026,47(3):163-171,9.基金项目
伊犁师范大学资源与生态研究所开放课题重点项目(YLNURE202201) （YLNURE202201）
伊犁哈萨克自治州科技计划项目(YYD2023A08). （YYD2023A08）
