食品工业科技2026，Vol.47Issue(5)：51-60,10.DOI:10.13386/j.issn1002-0306.2025070312
黑豆含硒肽对α-淀粉酶的抑制作用及其体外降血糖活性
Selenium-containing Peptides from Black Beans(Glycine max(L.)Merr.):Inhibition of α-Amylase and in Vitro Hypoglycemic Activity
摘要
Abstract
This study investigated whether selenium-containing peptides derived from selenium-rich black beans exert inhibitory effects on α-amylase and evaluated their potential hypoglycemic activity in vitro.Selenium-rich black beans from Guangxi,China,were used as raw materials,and selenium-containing peptides were screened and synthesized through a combination of single-factor experiments,response surface methodology,and peptidomics analysis.Four peptides—Se-MetLP,Se-MetIP,TLTSe-MetLR,and Se-CPSFGQ—were identified and evaluated for their α-amylase inhibitory activity.Among the four peptides,Se-MetIP exhibited the highest inhibitory effect,achieving an inhibition rate of 70.58%±2.21%at a concentration of 4 mg/mL,whereas TLTSe-MetLR showed the lowest half-maximal inhibitory concentration(IC50)of 0.47 mg/mL,indicating superior inhibitory potency.Based on their strong α-amylase inhibitory activity,Se-MetIP and TLTSe-MetLR were selected for further investigation of inhibition mechanisms using circular dichroism spectroscopy,isothermal titration calorimetry,and molecular docking analyses,and their in vitro hypoglycemic effects were subsequently evaluated in a hyperglycemic HepG2 cell model.Circular dichroism analysis revealed that peptide binding induced conformational changes in α-amylase,characterized by increased β-sheet content and reduced β-turn and random coil structures,suggesting alterations in the enzyme spatial conformation.Isothermal titration calorimetry demonstrated that peptide-enzyme interactions were exothermic.Molecular docking results showed that the binding free energies of Se-MetIP and TLTSe-MetLR with α-amylase were-5.399 and-6.401 kcal/mol,respectively,with van der Waals forces and hydrogen bonding as the dominant stabilizing interactions,complemented by hydrophobic effects.Furthermore,cellular assays demonstrated that Se-MetIP and TLTSe-MetLR significantly enhanced glucose consumption and promoted glycogen synthesis in insulin-resistant HepG2 cells,thereby improving glucose utilization.Overall,selenium-containing peptides from black beans effectively inhibit α-amylase activity and exhibit in vitro hypoglycemic potential,providing a theoretical basis for the development of hypoglycemic peptides from selenium-rich black beans.关键词
含硒肽/α-淀粉酶/分子对接/细胞实验/机制研究Key words
selenium-containing peptides/α-amylase/molecular docking/cell experiments/mechanism research分类
轻工纺织引用本文复制引用
徐颖,张雨,肖斌,车佳瑞,王欢,李东阳,曾桥,李儒仁..黑豆含硒肽对α-淀粉酶的抑制作用及其体外降血糖活性[J].食品工业科技,2026,47(5):51-60,10.基金项目
陕西省秦创原"科学家+工程师"队伍建设项目(2023KXJ156) （2023KXJ156）
西安市农业攻关项目(23NYGG0064) （23NYGG0064）
榆林市市县联动重大专项项目(2023-SXLD-5) （2023-SXLD-5）
陕北小杂粮优势特色产业集群项目. （）
