乳业科学与技术2026，Vol.49Issue(1)：1-8,8.DOI:10.7506/rykxyjs1671-5187-20251013-067
超声优化miR-26a脂质体与牛乳外泌体的递送效能对比分析
Comparative Analysis of the miR-26a Delivery Efficacy of Ultrasound-Treated Liposomes and Milk Exosomes
摘要
Abstract
Based on previous research suggesting that milk exosomes specifically express miR-26a,this study optimized the miR-26a encapsulation efficiency of liposomes through ultrasonic treatment,and it also evaluated the in vitro digestion performance of the ultrasonic-treated liposomes and compared its anti-inflammatory activity with that of natural milk exosomes.Single-factor experiments were conducted to optimize the molar charge ratio of cationic liposome vector to nucleic acid(N/P),ultrasonication time,and ultrasonic power for the preparation of miR-26a-encapsulated liposomes.The results demonstrated that when the N/P ratio was 4,ultrasonication time was 15 min,and ultrasonic power was 300 W,the encapsulation efficiency reached its peak of(89.51±1.37)%,with uniform particle size distribution and homogeneous morphology.In vitro simulated digestion experiments showed that the retention rate of miR-26a in the ultrasonic-treated liposomes(58%)was higher than that in the conventional liposomes(24%).Cell function experiments confirmed that the ultrasonic-treated liposomes not only enhanced the viability of normal Caco-2 cells but also effectively inhibited lipopolysaccharide(LPS)-induced inflammatory responses,significantly restoring cell viability under inflammatory conditions.Additionally,it reduced NO release and down-regulated the secretion of the inflammatory cytokines interleukin 6(IL-6),tumor necrosis factor α,and IL-1β,while effectively controlling the generation of reactive oxygen species.Notably,the ultrasonic-treated liposomes showed no significant differences from milk exosomes in terms of cell viability regulatory and inflammation inhibitory effects.In summary,this study demonstrates that ultrasonic can significantly improve the miR-26a delivery efficiency of liposomes.关键词
miR-26a/超声/脂质体/牛乳外泌体/抗炎活性Key words
miR-26a/ultrasound/liposomes/bovine milk exosomes/anti-inflammatory activity分类
轻工纺织引用本文复制引用
翟佳宁,原泽坤,陈佳慧,吕文佳,李艾黎,王凯丽..超声优化miR-26a脂质体与牛乳外泌体的递送效能对比分析[J].乳业科学与技术,2026,49(1):1-8,8.基金项目
国家自然科学基金面上项目(32272317) （32272317）
