食品科学2026，Vol.47Issue(3)：109-119,11.DOI:10.7506/spkx1002-6630-20230929-257
超高压辅助酶解制备胶原蛋白抗氧化肽及构效分析
Preparation and Structure-Activity Analysis of Antioxidant Peptides from Collagen by Ultra-high Pressure-Assisted Enzymatic Hydrolysis
摘要
Abstract
To improve the antioxidant capacity of bovine collagen-derived peptides and to identify novel peptides with antioxidant properties,this study employed ultra-high pressure(UHP)pretreatment followed by enzymatic hydrolysis with alkaline protease.The resulting peptides were analyzed for their antioxidant capacity,structural properties,molecular mass distribution,and amino acid sequences.It was found that UHP-assisted enzymatic hydrolysis increased the antioxidant activity and surface hydrophobicity of collagen peptides compared with conventional enzymatic hydrolysis,resulting in exposure of more hydrophobic groups and increasing hydrophobic amino acid content;morphological analysis showed that more amorphous structures were produced by UHP,resulting in smaller molecular masses and smaller peptide particles.Following isolation and purification,four novel antioxidant peptides with properties were identified.These findings provide theoretical support and technical reference for the production of food-derived antioxidants.关键词
超高压预处理/胶原蛋白抗氧化肽/结构特征/序列鉴定/分子对接Key words
ultra-high pressure pretreatment/collagen-derived antioxidant peptide/structural characterization/sequence identification/molecular docking分类
轻工纺织引用本文复制引用
王艳茹,王欣悦,何龙,余群力,张悦悦,李颖,徐瑾,张丽,韩广星..超高压辅助酶解制备胶原蛋白抗氧化肽及构效分析[J].食品科学,2026,47(3):109-119,11.基金项目
"十四五"国家重点研发计划重点专项(2021YFD1600200) （2021YFD1600200）
现代农业产业技术体系建设专项(CARS-37) （CARS-37）
2023年甘肃省研究生"创新之星"项目(2023CXZX-654) （2023CXZX-654）
