包装与食品机械2026，Vol.44Issue(1)：77-88,12.DOI:10.3969/j.issn.1005-1295.2026.01.009
微波辅助低共熔溶剂提取海带多糖的工艺优化及抗氧化活性分析
Process optimization and antioxidant activity analysis of microwave-assisted deep eutectic solvent extraction of laminaria japonica polysaccharides
摘要
Abstract
To explore the optimal extraction process for laminaria japonica polysaccharides(LJP)and to analyze their structural characteristics and antioxidant activity,a microwave-assisted deep eutectic solvent(DES)extraction method was employed.The optimal extraction process was obtained through single-factor experiments and response surface methodology.The extracted polysaccharides were structurally characterized using Fourier transform infrared spectroscopy(FT-IR),X-ray diffraction(XRD),and field emission scanning electron microscopy(FE-SEM),and their antioxidant activity was analyzed.The results showed that the optimal extraction conditions for LJP were:DES with 20%water content(betaine to urea molar ratio of 1:2),solid-to-liquid ratio of 1:40(g/mL),microwave power of 426 W,extraction temperature of 56℃,and extraction time of 60 min.Under these conditions,the polysaccharide yield reached 5.96%,which was 52.04%higher than that obtained by hot water extraction.FT-IR analysis indicated that the structure of LJP extracted by microwave-assisted DES was diverse.XRD analysis revealed that LJP was an amorphous substance.FE-SEM showed that hot water extraction and microwave-assisted DES extraction had different effects on the structure of LJP.The LJP extracted by microwave-assisted betaine-urea exhibited good antioxidant activity,with ABTS+• and DPPH radical scavenging rates of 83.40%and 62.34%,respectively.This study provides a theoretical basis for developing and optimizing the high-value utilization of laminaria japonica polysaccharides.关键词
海带多糖/低共熔溶剂/微波/抗氧化活性Key words
laminaria japonica polysaccharides/deep eutectic solvent/microwave/antioxidant activity分类
轻工纺织引用本文复制引用
郭丽,林宗伟,王喜庆,马雪,李杨,柴云雷,王鹏..微波辅助低共熔溶剂提取海带多糖的工艺优化及抗氧化活性分析[J].包装与食品机械,2026,44(1):77-88,12.基金项目
黑龙江省省属本科高等学校基本科研业务费基础研究项目(YWK10236210102) （YWK10236210102）
国家自然科学基金联合基金项目(U24A201282) （U24A201282）
