食品与发酵工业2026，Vol.52Issue(5)：121-131,11.DOI:10.13995/j.cnki.11-1802/ts.044477
联合宏基因组学及代谢组学探讨长双歧杆菌长亚种HSBL001后生元缓解结肠炎作用机制
Mechanism of the effect of postbiotic from Bifidobacterium longum subsp. longum HSBL001 on colitis based on integrated metagenomics and metabolomics
摘要
Abstract
Postbiotics are inactivated microorganisms or included their metabolites that benefit host health.Compared with probiotics,they have higher safety,stronger stability,and a broader range of action targets,thus showing significant advantages in alleviating inflam-matory bowel disease.This study evaluated the ameliorative effect of the postbiotic from Bifidobacterium longum subsp.longum HSBLOO1 on dextran sulfate sodium-induced ulcerative colitis mouse model,and explored the potential mechanism of this effect via metagenomics and metabolomics.Results indicate that HSBL001 significantly alleviates colitis-related symptoms,including reducing the disease activity index,increasing colon length,improving histopathological damage,regulating inflammatory cytokine secretion(increasing IL-10 while decreasing IL-6 and TNF-α),and alleviating splenomegaly.Metagenomic analysis showed that HSBL001 significantly increased the abun-dance of beneficial bacteria such as Duncaniella dubosii,Faecalibacterium rodentium,and Bacteroides thetaiotaomicron,while reducing the abundance of bacteria like Mucispirillum schaedleri and Pumilibacter muris.Metabolomic analysis revealed that HSBLO01 significant-ly upregulated 95 metabolites compared to the model group,including S-nitrosoglutathione,α-linolenic acid,and 7α-hydroxy-3-oxochol-est-4-en-27-oic acid,while significantly downregulating 80 metabolites including 3-hydroxyisovaleric acid and N-Acetyl-D-glucosaminitol.Pathway enrichment analysis of these differential metabolites shows that the postbiotic alleviates colitis mainly by regulating pathways such as primary bile acid biosynthesis and α-linolenic acid metabolism.In conclusion,supplementation with the HSBL001 effectively alleviates ulcerative colitis symptoms in mice.Its potential mechanisms are closely linked to regulating gut microbiota composition,increasing the a-bundance of bacteria such as D.dubosii and F.rodentium,as well as modulating primary bile acid biosynthesis and α-linolenic acid metab-olism.This study provides a new intervention method and research insights for alleviating colitis,and a theoretical basis for the industrial application of postbiotics.关键词
葡聚糖硫酸钠/炎症因子/胆汁酸代谢/溃疡性结肠炎/乳基后生元Key words
dextran sodium sulfate/inflammatory factors/bile acid metabolism/ulcerative colitis/milk-based postbiotic elements引用本文复制引用
刘建兵,程浩,熊艳霞,陈会军,殷丽丽,梅丽亚,张妍,谢有发,张阳..联合宏基因组学及代谢组学探讨长双歧杆菌长亚种HSBL001后生元缓解结肠炎作用机制[J].食品与发酵工业,2026,52(5):121-131,11.基金项目
江西省高价值专利培育项目(微生态大健康产品关键技术及产业化研究) （微生态大健康产品关键技术及产业化研究）
