食品与发酵工业2026，Vol.52Issue(5)：139-147,9.DOI:10.13995/j.cnki.11-1802/ts.042794
黄芪多糖对嗜酸乳杆菌CICC 22162冷冻干燥保护效果的研究
Cryoprotective effects of astragalus polysaccharides on freeze-drying survival of Lactobacillus acidophilus CICC 22162
摘要
Abstract
Astragalus polysaccharide(APS)exhibits multiple physiological activities,making its efficient development and utilization highly significant.This study investigated the cryoprotective effects of purified APS(composed of 64.38％glucose,19.81％galactose,and 15.81％arabinose)in combination with other cryoprotectants on Lactobacillus acidophilus CICC 22162 during freeze-drying.Results showed that the APS-based cryoprotectant significantly improved bacterial survival to(85.02±1.25)％.Scanning electron microscopy re-vealed more uniform cellular morphology,compared with conventional cryoprotectants,the APS-based formulation also resulted in a 15.5％lower than conventional protectants,lactate dehydrogenase leakage decreased by 38.7％,viability reduction in lysozyme sensitivi-ty tests was 20.6％lower,NaCl-induced membrane damage was reduced by 37.2％,and histone phosphorylation leakage decreased by 68％.These findings proved APS better maintains cell wall/membrane integrity,reduced intracellular content leakage,and enhanced re-sistance to environmental stresses.The formulation achieved 81.3％survival in simulated gastrointestinal digestion(33.9％improvement over conventional protectants)and maintained 9.18 lg CFU/mL viability after 120 days at4℃.The APS-based cryoprotectant enables production of freeze-dried probiotics with high viability,excellent gastrointestinal tolerance,and superior stability,representing an innova-tive protection strategy with significant application value in functional foods and gut microbiota regulation.关键词
黄芪多糖/冷冻干燥保护剂/嗜酸乳杆菌/存活率/细胞完整性/稳定性Key words
astragalus polysaccharides/freeze drying protectant/Lactobacillus acidophilus/survival rate/cell integrity/stability引用本文复制引用
杜建明,张珍,尚琪,王学瑞,史建莉,宋乐,谢鹏,蔡毓涵..黄芪多糖对嗜酸乳杆菌CICC 22162冷冻干燥保护效果的研究[J].食品与发酵工业,2026,52(5):139-147,9.基金项目
甘肃省农业农村厅科技支撑项目(KJZC-2025-27) （KJZC-2025-27）
中国乳业协会利乐专项基金(CDIAKCJJ-TP-2024-008) （CDIAKCJJ-TP-2024-008）
甘肃省产业支撑项目(2023CYZC-43) （2023CYZC-43）
甘肃省现代寒旱特色农业药产业技术体系(GSARS06-06) （GSARS06-06）
