食品与发酵工业2026，Vol.52Issue(6)：303-309,中插18-中插21,11.DOI:10.13995/j.cnki.11-1802/ts.043234
黄水肽的分离鉴定及其潜在的血管紧张素转换酶抑制活性
Isolation and identification of Huangshui peptides and their potential inhibitory activity of angiotensin-converting enzyme
廖勤俭 1王芳 1李芹 1郭艳 1杨紫丹 1杨皓 1周韩玲 1苏建 2郑佳2
作者信息
- 1. 宜宾五粮液股份有限公司,四川宜宾,644007
- 2. 宜宾五粮液股份有限公司,四川宜宾,644007||固态发酵资源利用四川省重点实验室,四川宜宾,644007
- 折叠
摘要
Abstract
Huangshui is a by-product generated during the Baijiu brewing process,and its resource utilization rate remains relatively low.In this study,high-value-added bioactive peptides were isolated and identified from Huangshui,and their potential angiotensin-con-verting enzyme(ACE)inhibitory activities were investigated to explore a novel approach for the resource utilization of Huangshui.A pep-tide fraction M3(molecular weight 0.15-1 kDa),characterized by high peptide content and ACE inhibitory activity,was prepared through organic solvent precipitation and membrane filtration.Subsequently,the M3 components were separated using macroporous resin column chromatography,and 61 peptides were identified via high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(HPLC/QTOF).The virtual screening of ACE inhibitory peptides using molecular docking revealed that the fol-lowing peptides exhibited promising inhibitory activity:Val-Tyr,Cyclo(Arg-Pro),Cyclo(Glu-Leu),Cyclo(Pro-Leu),Cyclo(Pro-Tyr),Cyclo(Leu-Arg),Pro-Val-Ala,and Pro-Val-Val.The binding energies between these eight peptides with the ACE target enzyme were all lower than-7 kcal/mol.These peptides can form hydrogen bonds and hydrophobic interactions with the residues in the ACE active cen-ter,thereby stabilizing the complex formation.This indicates their potential as effective ACE inhibitory peptides.This study successfully i-solated and identified 61 small peptides from Huangshui for the first time and screened out potential high-activity ACE inhibitory peptides through molecular docking.These findings provide valuable technical references for the development and utilization of Huangshui peptides,thereby promoting energy conservation and emission reduction in Baijiu production enterprises.关键词
黄水肽/分离鉴定/分子对接/血管紧张素转换酶/抑制Key words
Huangshui peptides/isolation and identification/molecular docking/angiotensin-converting enzyme/inhibition引用本文复制引用
廖勤俭,王芳,李芹,郭艳,杨紫丹,杨皓,周韩玲,苏建,郑佳..黄水肽的分离鉴定及其潜在的血管紧张素转换酶抑制活性[J].食品与发酵工业,2026,52(6):303-309,中插18-中插21,11.
