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菌群强化对大曲微生物群落结构及代谢影响的研究

张毅 严伟 黄钧 王加彬 金垚 白玉光 张敏 周荣清

食品与发酵工业2026,Vol.52Issue(6):317-327,11.
食品与发酵工业2026,Vol.52Issue(6):317-327,11.DOI:10.13995/j.cnki.11-1802/ts.043477

菌群强化对大曲微生物群落结构及代谢影响的研究

Study on the effects of microbial fortifying on the microbial community structure and metabolism of Daqu

张毅 1严伟 2黄钧 1王加彬 2金垚 1白玉光 2张敏 2周荣清3

作者信息

  • 1. 四川大学轻工科学与工程学院,四川 成都,610065
  • 2. 国家固态酿造工程技术研究中心河套联合研究中心,内蒙古巴彦淖尔,015400||内蒙古河套酒业集团股份有限公司,内蒙古巴彦淖尔,015400
  • 3. 四川大学轻工科学与工程学院,四川 成都,610065||国家固态酿造工程技术研究中心河套联合研究中心,内蒙古巴彦淖尔,015400||内蒙古河套酒业集团股份有限公司,内蒙古巴彦淖尔,015400
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摘要

Abstract

This study employed multiphase detection technology to investigate the effects of adding 0.2%(M2)and 0.4%(M4)Muqu on the physicochemical properties,microbial community structure,and metabolite profile of Daqu.The results demonstrated that,compared to the control samples(M0),the liquefaction and saccharification abilities of M2 decreased by 3.44%and 19.05%,respec-tively.In contrast,the amino nitrogen content increased significantly(96.21%),and the acetic acid content also increased by 65.11%,while oxalic acid levels dropped below the detection limit.Furthermore,the addition of Muqu,substantially elevated the concentration of acid,pyrazines,and heterocyclic substances.Microbial community analysis revealed that Muqu altered the dominant bacterial community position:the bacterial community shifted from a single dominance of Saccharopolyspora to a co-dominant community characterized by Ther-moactinomyces,Saccharopolyspora,and Weissella.The fungal community evolved from a single dominance of Thermomyces to a co-domi-nance of Thermomyces and Thermoascus.Mechanistic analysis of community assembly indicated that bacterial communities were governed primarily by deterministic processes,whereas fungal communities stochastic assembly in M2 before shifting to deterministic processes in M4.These results provide a theoretical foundation for developing microbial targeted strategies of medium-high-temperature Daqu.

关键词

母曲强化/代谢标志物/代谢途径/理化性质/群落聚集

Key words

Muqu fortification/metabolic markers/metabolite pathway/physicochemical properties/community assembly

引用本文复制引用

张毅,严伟,黄钧,王加彬,金垚,白玉光,张敏,周荣清..菌群强化对大曲微生物群落结构及代谢影响的研究[J].食品与发酵工业,2026,52(6):317-327,11.

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