食品与发酵工业2026，Vol.52Issue(6)：231-237,7.DOI:10.13995/j.cnki.11-1802/ts.043346
耐酸性食窦魏斯氏菌的诱变选育及机制分析
Mutagenic breeding and mechanism analysis of acid-tolerant Weissella cibaria
摘要
Abstract
Weissella cibaria demonstrates beneficial effects on gut health and the host immune response.To select acid-tolerant strains of W.cibaria,in this study,W.cibaria W25 screened from kimchi products was used as the starting strain.Through ultraviolet mutagenesis(UV,60 s),ethyl methanesulfonate mutagenesis(EMS,0.5％,45 min),as well as composite mutagenesis,including sequential treat-ments:UV followed by EMS(UV-EMS)and EMS followed by UV(EMS-UV),four mutants,namely U20,E7,U-E18 and E-U28,were obtained.U-E18 showed the best performance,and its acid production activity was significantly higher than that of W25.In the simulation of the tolerance to the gastrointestinal environment,the survival rate of U-E18 in gastric fluid with a pH of 1.5 was 20.92％,and the sur-vival rate in the intestinal fluid environment after 8 hours was 13.78％,W25 was completely inactivated.The growth rate of U-E18 in the presence of bile salts after 8 hours reached 32.48％,which was much higher than the 18.36％of W25.Functional genomics analysis re-vealed the molecular mechanism of acid tolerance of U-E18.Functional annotation analysis based on the cluster of orthologous groups of proteins(COG)revealed significant enrichment in pathways related to carbohydrate transport and metabolism,translation and ribosome biogenesis,as well as amino acid transport and metabolism.KEGG pathway analysis identified the ABC transporter system as playing a pivotal role.These findings establish U-E18 as an exceptional acid-tolerant microbial resource,offering substantial potential for food fer-mentation research and industrial applications.关键词
食窦魏斯氏菌/耐酸性/诱变选育/生长特性/功能基因Key words
Weissella cibaria/acid tolerance/mutagenic breeding/growth characteristics/functional genomics引用本文复制引用
刘莞莹,曾玲,曲彦颖,陈菲琳,朴泓洁,刘长蕾,文宇萍,金清..耐酸性食窦魏斯氏菌的诱变选育及机制分析[J].食品与发酵工业,2026,52(6):231-237,7.基金项目
吉林省科技发展计划技术创新引导项目(20220402053GH) （20220402053GH）
延边大学科技发展计划项目(ydkj202405) （ydkj202405）
