食品与发酵工业2026，Vol.52Issue(6)：246-253,8.DOI:10.13995/j.cnki.11-1802/ts.044197
抗菌肽Andricin 01对金黄色葡萄球菌生物被膜形成的抑制作用
Inhibitory effect of antimicrobial peptide Andricin 01 on biofilm formation of Staphylococcus aureus
摘要
Abstract
To study the inhibitory effect of Andricin 01,an antimicrobial peptide isolated from the skin mucus of the giant salamander in the laboratory,on the biofilm of Staphylococcus aureus ATCC25923,the minimum inhibitory concentration(MIC)of Andricin 01 against the strain was determined by the microdilution method.The effects of Andricin 01 on the membrane integrity and permeability of Staphylo-coccus aureus were analyzed by flow cytometry and alkaline phosphatase assay.The inhibitory ability of Andricin 01 on the biofilm of S.au-reus ATCC25923 was observed by crystal violet staining,scanning electron microscopy and confocal laser scanning microscopy.The results showed that the MIC of Andricin 01 against S.aureus ATCC25923 was 16 μg/mL,and it caused the death of the bacteria by altering per-meability of the cell membrane.The results of crystal violet staining indicated that the inhibition rate of Andricin 01 at 3/4 MIC concentra-tion on the biofilm of S.aureus was more than 50％.The colony count experiment demonstrated that sub-inhibitory concentrations of Andri-cin 01 could effectively reduce the number of viable bacteria in the biofilm structure.Scanning electron microscopy revealed that the struc-ture of the S.aureus ATCC25923 biofilm treated with Andricin 01 was disrupted and the distribution was loose.Observation by laser confo-cal scanning microscope revealed that the fluorescence signal intensity of the Staphylococcus aureus ATCC25923 biofilm treated with Andri-cin 01 was diminished,the number of viable bacteria was scarce,the colony distribution was sparse and not dense,the amount of mature plaque was small,and the membrane thickness was relatively thin.This study initially revealed the inhibitory effect of the skin mucus anti-microbial peptide Andricin 01 from the giant salamander on the biofilm of S.aureus,providing a novel perspective for the prevention and control of biofilm contamination caused by foodborne pathogen Staphylococcus aureus.关键词
金黄色葡萄球菌/抗菌肽/Andricin 01/生物被膜Key words
Staphylococcus aureus/antimicrobial peptide/Andricin 01/biofilm引用本文复制引用
任婷,刘晓影,黄怡婷,毕芳芳,胡仲秋,裴金金..抗菌肽Andricin 01对金黄色葡萄球菌生物被膜形成的抑制作用[J].食品与发酵工业,2026,52(6):246-253,8.基金项目
陕西省自然科学基础研究计划一般项目(面上)(2025JC-YBMS-962) （面上）
