中外葡萄与葡萄酒Issue(2)：10-17,8.DOI:10.13414/j.cnki.zwpp.2026.02.002
不同发病程度'木纳格'葡萄酸腐病微生物群落差异研究
Study on Microbial Community Differences in'Munage'Grape Sour Rot Disease at Different Disease Severities
摘要
Abstract
To reveal the variation patterns of microbial communities in'Munage'grapes under different degrees of sour rot disease severity,this study employed high-throughput sequencing technology to analyze bacterial and fungal communities in healthy and mildly,moderately,and severely infected fruits.Results showed that microbial α-diversity on fruit surfaces significantly decreased with increasing disease severity.In bacterial communities,the abundance of Proteobacteria and Firmicutes increased,Lactobacillus showed exponential growth,and Acetobacter became the dominant bacterial group.Fungal community diversity also significantly decreased,with Hanseniaspora abundance gradually declining as disease severity increased,while Saccharomycodes and Starmerella abundance increased significantly.LEfSe analysis identified characteristic biomarkers at each disease stage:for bacteria,Pseudomonas was identified in healthy fruits,Acetobacter group in mildly infected fruits,Fructobacillus in moderately infected fruits,and Lactobacillus in severely infected fruits;for fungi,Pichia was identified in healthy fruits,Hanseniaspora in mildly infected fruits,Hanseniaspora uvarum and Kazachstania in moderately infected fruits,and Saccharomycodes in severely infected fruits.This study systematically revealed the differences in microbial communities of'Munage'grape sour rot disease under different disease severities,providing a scientific basis for constructing precision prevention and control systems based on microbial ecological regulation.关键词
'木纳格'葡萄/酸腐病/微生物群落/高通量测序/群落差异Key words
'Munage'grapes/sour rot disease/microbial community/high-throughput sequencing/community differences分类
农业科技引用本文复制引用
应泽威,李克梅,陈浩宇,段佳辰,郝敬喆,白剑宇,张雯..不同发病程度'木纳格'葡萄酸腐病微生物群落差异研究[J].中外葡萄与葡萄酒,2026,(2):10-17,8.基金项目
中央财政林草科技推广示范项目(新[2024]TG14号)财政部和农业农村部:国家葡萄产业技术体系(CARS-29) （新[2024]TG14号）
