现代食品科技2026，Vol.42Issue(2)：11-20,10.DOI:10.13982/j.mfst.1673-9078.2026.2.1575
雨生红球藻粉通过改善线粒体功能障碍缓解尿路梗阻导致的肾纤维化
Haematococcus pluvialis Powder Alleviates Urinary Tract Obstruction-induced Renal Fibrosis by Ameliorating Mitochondrial Dysfunction
摘要
Abstract
The potential of Haematococcus pluvialis powder in alleviating urinary tract obstruction-induced renal fibrosis by ameliorating mitochondrial dysfunction and epithelial-mesenchymal transition(EMT)was investigated in this study.Various methods,including the CCK8 assay,immunofluorescence,mitochondrial membrane potential measurement,mitochondrial reactive oxygen species detection assay,and immunohistochemical staining,were used for validation.The results demonstrated the ability of H.pluvialis powder in mitigating renal pathological changes in rats induced by unilateral ureteral obstruction(UUO)surgery.Compared with the model group,treatment with H.pluvialis powder significantly decreased fibronectin 1 expression(65.52％).In vitro,H.pluvialis powder ameliorated transforming growth factor-β1-induced changes in EMT markers in HK-2 cells,with the positive area fraction of E-cadherin increasing by 51.35％,and those of N-cadherin and vimentin decreasing by 62.30％and 39.36％,respectively.An increase in mitochondrial membrane potential and a decrease in mitochondrial superoxide accumulation were also observed.Subsequently,astaxanthin(AST)was used to intervene in the rat UUO and HK-2 pro-fibrotic cell models.The results revealed that H.pluvialis powder outperformed AST at equivalent dosages/concentrations.In summary,this study found that H.pluvialis powder could alleviate the pathological progression of renal fibrosis caused by urinary tract obstruction via mechanisms that include improving extracellular matrix deposition,reducing renal cortical injury,modulating the EMT process,and mitigating mitochondrial dysfunction.Therefore,H.pluvialis powder shows potential for the development of functional foods with renoprotective effects.关键词
雨生红球藻粉/虾青素/尿路梗阻/肾纤维化/线粒体功能障碍/上皮间质转化Key words
Haematococcus pluvialis powder/astaxanthin/urinary tract obstruction/renal fibrosis/mitochondrial dysfunction/epithelial-mesenchymal transition引用本文复制引用
吴雨桐,陈彩炼,谢金梅,张勇,郑云剑,常秀亭,胡文婷,谢曦..雨生红球藻粉通过改善线粒体功能障碍缓解尿路梗阻导致的肾纤维化[J].现代食品科技,2026,42(2):11-20,10.基金项目
海南省自然科学基金项目(823RC467 （）
821MS027 （）
822MS053) （）
国家自然科学基金项目(82360731) （82360731）
海南大学全健康协同创新中心项目(XTCX2022JKB08) （XTCX2022JKB08）
海南省研究生创新科研课题(Qhys2024-284) （Qhys2024-284）
云南省孙树侠专家工作站(202305AF150114) （202305AF150114）
