中国酿造2026，Vol.45Issue(3)：84-90,7.DOI:10.11882/j.issn.0254-5071.2026.03.012
高产γ-氨基丁酸菌株的筛选鉴定及其在清香型白酒中的应用
Screening and identification of high-yield γ-aminobutyric acid strains and their application in light-flavor Baijiu
摘要
Abstract
High-yield γ-aminobutyric acid(GABA)strains were isolated and screened from fermented grains by traditional culture isolation method combined with HPLC-MS,and identified by morphological observation and molecular biological techniques,the superior strain with high-yield GABA was screened by environmental tolerance analysis,the fermentation process for GABA production was optimized by single factor experiments,and it was applied to small-scale brewing experiment of light-flavor(Qingxiangxing)Baijiu.The results showed that 2 strainswith high-yield GABA were screened and identified,which were Lentilactobacillus hilgardii X920 and Lentilactobacillus diolivorans X952.Among them,L.hilgardii X920 was a superior strain with high-yield GABA,which had good environmental tolerance.It could withstand temperature 47 ℃,pH 4.0,and ethanol volume fraction 12％.The optimized fermentation process for GABA production by L.hilgardii X920 was as follows:using MRS broth as the fermentation substrate supplemented with L-glutamate 50 g/L and glucose 20 g/L,incubation at 37℃ for 3 d.Under these conditions,GABA yield reached 29.53 g/L,which was 19.5 times higher than that before optimization.Adding 2‰ fermentation liquid of L.hilgardii X920(cell concentration 1×109 CFU/L)during the fermentation stage of light-flavor Baijiu could increase the GABA content in the fermented grains by 32 times,with the highest content reaching 0.48 g/kg.This research not only established a complete system for screening and fermentation process optimization of high-yield GABA strains,but also provided feasible process scheme for achieving targeted enrichment of GABA in fermented grains,and had significant industrial application value.关键词
酒醅/γ-氨基丁酸/筛选/鉴定/耐受性/发酵工艺优化Key words
fermented grains/γ-aminobutyric acid/screening/identification/tolerance/fermentation process optimization分类
轻工纺织引用本文复制引用
陈凯,朱丽萍,江威,熊晓通,杨强,陈申习..高产γ-氨基丁酸菌株的筛选鉴定及其在清香型白酒中的应用[J].中国酿造,2026,45(3):84-90,7.基金项目
湖北省重点研发计划(2023BBB004) （2023BBB004）
