中国食物与营养2026，Vol.32Issue(3)：15-20,6.
高效液相色谱法测定芝麻油中芝麻素的含量
Determination of Sesamin Content in Sesame Oil by High-Performance Liquid Chromatography(HPLC)
摘要
Abstract
[Objective]To establish a high-performance liquid chromatography(HPLC)method for accurate and rapid determination of sesamin content in sesame oil.[Method]The analysis was performed using an XSelect CSH C18 chromatographic column(4.6 mm×250 mm,5 μm).The mobile phase consisted of methanol-water(volume ratio 80:20,V:V).at a flow rate of 0.8 mL/min.The detection wavelength was set at 287 nm,the column temperature was maintained at 35 ℃,and the injection volume was 10 μL.[Result]The linear regression equation for sesamin was Y=0.000 08X-1.437 9(Y:peak area;X:concentration,μg/mL),with a correlation coefficient(R²=0.995 1).The linear range was 4.987 008 μg/mL to 149.702 4 μg/mL.The spike recovery test showed an average recovery rate of 101.03%,indicating good method precision.[Conclusion]This method is simple to operate,highly sensitive,and exhibits good repeatability.It can accurately determine the sesamin content in sesame oil,making it suitable for quality control and related analyses of sesame oil.关键词
高效液相色谱法/芝麻油/芝麻素Key words
high performance liquid chromatography(HPLC)/sesame oil/sesamin引用本文复制引用
洋西吉,吴宗耀,占堆..高效液相色谱法测定芝麻油中芝麻素的含量[J].中国食物与营养,2026,32(3):15-20,6.基金项目
2024年度藏医药标准化研究项目(西藏地方藏医特色食材目录编制研究,项目编号:ZYBZ2025002). （西藏地方藏医特色食材目录编制研究,项目编号:ZYBZ2025002）
