粮油食品科技2026，Vol.34Issue(2)：41-49,9.DOI:10.16210/j.cnki.1007-7561.2026.02.006
基于非靶向代谢组学分析两种不同制油方法提取的核桃油脂成分差异
Analysis of Differential Components in Walnut Oil Extracted by Two Different Methods Based on Non-targeted Metabolomics
摘要
Abstract
Walnut oil produced by aqueous extraction has the characteristics of higher endogenous antioxidant components,better quality and storability compared with traditional methods.To further study its liposoluble components,a non-targeted metabolomics study was conducted on walnut oil extracted by two methods(hydraulic pressing and aqueous extraction)using ultra performance liquid chromatography-mass spectrometry.The results showed that there are no significant difference in the physicochemical indicators of walnut oil processed by the two methods.A total of 1 006 components were identified in the walnut oil,mainly including lipids and lipid-like molecules(40.56％),phenylpropanoids and polyketides(18.19％),organic acids and derivatives(10.83％),etc.There were a total of 142 different components in the two types of walnut oil.Compared with the hydraulic oil,82 components were upregulated and 60 components were downregulated of aqueous extracted oil.The metabolic pathways with significant differences had five types:fatty acid metabolism,flavonoid biosynthesis,phenylpropanoid biosynthesis,cutin/suberine/wax biosynthesis,ubiquinone/other terpenoid-quinone biosynthesis.There were a total of 27 key differential components in metabolic pathways,mainly including oxidized fatty acids,polyphenols,tocopherols,branched fatty acid esters of hydroxy fatty acids(FAHFA),etc.In conclusion,compared with the hydraulic oil,the aqueous extracted oil contains more lipid-soluble functional components.关键词
核桃油/水代核桃油/非靶向代谢组学/差异成分/代谢通路Key words
walnut oil/aqueous extracted walnut oil/non-targeted metabolomics/differential components/metabolic pathways分类
轻工纺织引用本文复制引用
缪福俊,徐田,李文玕,马婷,耿树香,宁德鲁..基于非靶向代谢组学分析两种不同制油方法提取的核桃油脂成分差异[J].粮油食品科技,2026,34(2):41-49,9.基金项目
云南省基础研究计划面上项目"深纹核桃水代制油过程中多酚迁移及油脂氧化酸败调控的机理研究"(202401AT070300) （202401AT070300）
云南省"兴滇英才支持计划"青年人才项目(XDYC-QNRC-2024-458) Yunnan Fundamental Research Projects"Research on the Polyphenol Migration in the Aqueous Oil Extraction Process and the Mechanism of Oil Oxidative Rancidity in Juglans sigillata"(No.202401AT070300) （XDYC-QNRC-2024-458）
Young Talent Program of the"Xingdian Talent Support Initiative"of Yunnan Province(No.XDYC-QNRC-2024-458) （No.XDYC-QNRC-2024-458）
