食品工业科技2026，Vol.47Issue(8)：100-108,9.DOI:10.13386/j.issn1002-0306.2025040212
中亚白及中性多糖的结构解析及抗炎活性研究
Structural Elucidation and Anti-inflammatory Activity of Neutral Polysaccharide from Orchis chusua D.Don
摘要
Abstract
To systematically investigate the structural features and anti-inflammatory activity of neutral polysaccharide(USP-N)from Orchis chusua D.Don,a medicinal and edible plant was selected.The structure of USP-N was comprehensively elucidated through partial acid hydrolysis,Smith degradation,methylation analysis,and 2D-NMR spectroscopy.Its anti-inflammatory effects were evaluated using a zebrafish inflammation model.Structural analysis revealed that the main chain of USP-N was composed of alternating β-1,4-linked glucose(38.5%)and mannose(33.8%),forming a glucomannan backbone.The side chains contained small amounts of rhamnose,arabinose,and galactose,with some mannose residues featuring 2-O-acetyl groups.Bioactivity assays demonstrated that USP-N treatment significantly suppressed LPS-induced nitric oxide(NO)release(P<0.0001)and inhibited neutrophil migration to the tail injury site in zebrafish(P<0.0001).Furthermore,dose-dependent reductions in neutrophil and macrophage recruitment to the caudal region following mechanical injury were observed.This study provides a theoretical foundation for applying Orchis chusua D.Don polysaccharides in functional foods and pharmaceutical development.关键词
中亚白及/多糖/甲基化/二维核磁/抗炎活性Key words
Orchis chusua D.Don/polysaccharide/methylation/2D NMR/anti-inflammatory activity分类
轻工纺织引用本文复制引用
杨智艳,王宇帆,阿依左克拉木·牙森,王艳丽,魏瑞杰,顾雨露,热合巴提·努尔夏提,胡尔西丹·伊麻木..中亚白及中性多糖的结构解析及抗炎活性研究[J].食品工业科技,2026,47(8):100-108,9.基金项目
中央引导地方科技发展资金项目(ZYYD2025CG18) （ZYYD2025CG18）
新疆维吾尔自治区"天池英才"引进计划(2023TCYCQNBS03) （2023TCYCQNBS03）
棉籽残渣多糖的活性成分研究(S202410764029). （S202410764029）
