食品工业科技2026，Vol.47Issue(8)：143-152,10.DOI:10.13386/j.issn1002-0306.2025040317
低温等离子体诱导乳清分离蛋白-壳聚糖复合颗粒的结构特性及其花青素负载性能
Structural Characteristics and Anthocyanin Loading Performance of Whey Protein Isolate-Chitosan Composite Particles Induced by Cold Plasma
摘要
Abstract
To investigate the inducing effect of dielectric barrier discharge cold plasma(DBD-CP)technology(treatment voltages:30,90,150 kV,treatment times:30,60,90 s)on the conformational changes of whey protein isolate(WPI)-chitosan(CS)composite particles and its regulatory effect on the loading performance of anthocyanins(ACNs),this study employed a two-factor full factorial experimental design.By systematically evaluating the encapsulation efficiency(EE),loading capacity(LC),ζ-potential,and particle size distribution of the composite particles for ACNs,the optimal DBD-CP treatment parameters were screened.Fourier transform infrared spectroscopy(FTIR),X-ray diffraction(XRD),scanning electron microscopy(SEM),fluorescence spectroscopy(FS),and circular dichroism(CD)were utilized as multi-scale characterization techniques to deeply analyze the DBD-CP-induced molecular conformational changes in WPI-CS.Based on the results of the above systematic evaluation,the optimal DBD-CP treatment parameters were screened and determined as voltage 90 kV and time 60 s.Under this optimal parameter set,the EE and LC of ACNs increased to 91.93%and 4.59%,respectively,significantly higher than those of the untreated group(P<0.05).The ζ-potential increased,enhancing electrostatic repulsion between particles and significantly improving system stability.Particle size analysis showed that distribution uniformity and particle dispersibility were improved.Structural characterization revealed that:Reactive species(e.g.,electrons,ions)in DBD-CP promoted the exposure of hydrophobic groups in WPI and decreased the deacetylation degree of CS through oxidative modification,thereby enhancing the formation of hydrogen bonding and electrostatic cross-linking networks,simultaneously,the plasma etching effect optimized the pore uniformity of the complex,forming a dense encapsulation structure.DBD-CP treatment effectively modulated the structure of WPI-CS composite particles through synergistic effects(oxidative modification induced by reactive species and the etching effect),significantly improving their loading performance for ACNs and the system stability.This provides a theoretical basis for the application of cold plasma technology in stabilized delivery systems for bioactive components.关键词
低温等离子体/乳清分离蛋白/壳聚糖/花青素/负载性能Key words
cold plasma/whey protein isolate/chitosan/anthocyanin/loading performance分类
轻工纺织引用本文复制引用
许博文,石翀,周丹丹,王意程,李维林,方东路..低温等离子体诱导乳清分离蛋白-壳聚糖复合颗粒的结构特性及其花青素负载性能[J].食品工业科技,2026,47(8):143-152,10.基金项目
国家重点研发计划项目(2024YFD2101003) （2024YFD2101003）
江苏省优秀青年基金项目(BK20240140) （BK20240140）
南京林业大学瑞华经济林研究院经济林创新研究项目(510010003). （510010003）
