食品工业科技2026，Vol.47Issue(8)：153-161,9.DOI:10.13386/j.issn1002-0306.2025040349
刺梨纳米囊泡的制备、表征及其抗氧化、降血糖与抗毒溶血活性研究
Preparation,Characterization of Rosa roxburghii Tratt.Nanovesicles and Its Antioxidant,Hypoglycemic,and Antihemolytic Activities
摘要
Abstract
To deeply investigate the structural characteristics and activities of nanovesicles derived from Rosa roxburghii Tratt.(RDNs),they were isolated,extracted,and characterized.Additionally,their antioxidant,hypoglycemic,and anti-hemolytic activities against Staphylococcus aureus exotoxin were explored.The RDNs were isolated using ultracentrifugation,and their morphology was characterized through transmission electron microscopy(TEM).The protein,lipid,and RNA composition/concentrations were quantified employing the BCA assay,thin-layer chromatography(TLC),and RNA extraction kits,respectively.The antioxidant capacity was assessed using DPPH and ABTS+radical scavenging assays,while the hypoglycemic activity was evaluated through α-glucosidase and α-amylase inhibition assays.Using 2%rat erythrocytes as the research object,the experiment was divided into blank control group,positive control group,S.aureus-EVs group,RDNs group,and RDNs+S.aureus-EVs group,and in vitro anti-hemolytic rate of RDNs was detected.The results indicated that RDNs had an average hydrodynamic diameter of 155.27±1.39 nm,a Zeta potential of −8.60±0.64 mV,and maintained stability at −80℃for 12 days.The particles remained stable during the first 6 days of storage at 4℃,but exhibited significant particle size changes after surpassing the 6-day mark(P<0.01).Particle size remained unchanged(P>0.05)at pH1.2,while pH6.5 showed significant change in particle size(P<0.05).Biochemical analysis uncovered the presence of protein(5.50±0.00 mg/mL),lipid(4.23±0.40 mg/mL),and RNA(3.91±1.97 μg/mL).Notably,protein bands were observed at 24,50~70,and 70 kDa.Lipid components exhibited an intricate composition,which included phytosterols and lecithins.Concentration-dependent(0~212.30 mg/mL)scavenging of DPPH and ABTS+radicals was observed,with the half-inhibitory concentrations(IC50)for the two radicals being 112.90 and 64.38 mg/mL,respectively.Additionally,when the concentration reached 212.30 mg/mL,the RDNs were capable of eliminating 99.80%of ABTS+.The α-glucosidase and α-amylase inhibition rates were 48%and 21%,respectively,significantly lower than positive controls(P<0.01).The hemolysis rates were nearly 0 in the RDNs group,64%in the S.aureus-EVs group,and 8%in the RDNs+S.aureus-EVs group,which was significantly lower than that in the S.aureus-EVs group(P<0.01).The RDNs had antioxidant and hypoglycemic activities and could exert anti-hemolytic effects against S.aureus exotoxins,laying a foundation for their potential applications in the fields of antioxidants and natural antibacterial agents.关键词
刺梨/纳米囊泡/生物活性/金黄色葡萄球菌/外毒素Key words
Rosa roxburghii Tratt./nanovesicles/bioactivity/Staphylococcus aureus/exotoxin分类
轻工纺织引用本文复制引用
曹明未,庞玉新,魏永耀,王甜甜,邓芳雪,刘炳楠,刘畅..刺梨纳米囊泡的制备、表征及其抗氧化、降血糖与抗毒溶血活性研究[J].食品工业科技,2026,47(8):153-161,9.基金项目
国家中医药管理局高水平重点学科建设项目(zyyzdxk-2023186) （zyyzdxk-2023186）
贵州省正品药材种质资源库设备建设项目(正品药材优势特色产业集群(中药材产业集群))(3411-411000163) （正品药材优势特色产业集群(中药材产业集群）
贵州中医药大学喀斯特药用资源保护与创新利用科技创新人才团队项目(GZYTDHezi[2022]001) （GZYTDHezi[2022]001）
贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY-2025-071). （QZYY-2025-071）
