食品科学技术学报2026，Vol.44Issue(2)：135-145,220,12.DOI:10.12301/spxb202600023
硒化金耳多糖的制备、结构表征及生物活性
Preparation,Structural Characterization,and Biological Activities of Selenized Tremella aurantialba Polysaccharides
摘要
Abstract
To investigate the structural characteristics and biological activity differences between Tremella aurantialba polysaccharides(TAP)and selenized Tremella aurantialba polysaccharides(Se-TAP),TAP was prepared using liquid fermentation technology and purified through ethanol precipitation,Sevage deproteinization,and membrane separation.Se-TAP was synthesized by reacting TAP with sodium selenite and nitric acid at 80℃for 7 h.Structural characterization results indicated that both TAP and Se-TAP were primarily composed of glucose and mannose,featuring a β-type glycosidic linkage and a triple-helix structure.Characteristic absorption peaks for Se-TAP were observed in the infrared spectrum at 850 cm-1and 750 cm-1,confirming the successful introduction of selenite ester groups(Se=O and C—O—Se),with a measured selenium content of 533.44 μg/g.Due to acid hydrolysis in the reaction system,Se-TAP underwent side-chain trimming,with the weight-average molecular weight decreasing from 4.62×106 Da(TAP)to 3.94×106 Da,and the PDI decreasing from 2.22 to 2.03,indicating a more uniform molecular weight distribution.In vitro bioactivity assays demonstrated that Se-TAP exhibited significantly enhanced antioxidant capacity compared to TAP,with improved scavenging rates against DPPH,ABTS+,and hydroxyl radicals,as well as increased total reducing power.In terms of antitumor activity,Se-TAP showed dose-dependent inhibitory effects on gastric cancer(HGC-27),cholangiocarcinoma(RBE),and pancreatic cancer(PANC-1)cells.Notably,against HGC-27 cells,Se-TAP achieved an IC50 value of 652.95 μg/mL and an inhibition rate of 58.60％at a concentration of 1 000 μg/mL,outperforming the positive control 5-fluorouracil.Fluorescence staining confirmed that its mechanism involved inducing cancer cell apoptosis.The findings indicated that selenization modification retained the backbone structure of TAP while significantly enhanced their antioxidant and antitumor activities through structural optimization.This study aimed to provide a theoretical reference for expanding the application of TAP and Se-TAP in the functional food sector.关键词
金耳多糖/硒化修饰/结构表征/抗氧化活性/抗肿瘤活性Key words
Tremella aurantialba polysaccharide/selenization modification/structural characterization/antioxidant activity/antitumor activity分类
轻工纺织引用本文复制引用
朱鹏,顾秋亚,吕蓓蓓,余晓斌..硒化金耳多糖的制备、结构表征及生物活性[J].食品科学技术学报,2026,44(2):135-145,220,12.基金项目
轻工技术与工程国家一级学科项目(LITE2018-11). National First-Class Discipline Project of Light Industry Technology and Engineering(LITE2018-11). （LITE2018-11）
