食品科学2026，Vol.47Issue(5)：162-173,12.DOI:10.7506/spkx1002-6630-20250922-171
膨腹海马乙醇提取物改善小鼠高尿酸血症的作用机制
Molecular Mechanism of Ethanol Extract from Hippocampus abdominalis in Improving Hyperuricemia in Mice
摘要
Abstract
Objective:To study the effect and molecular mechanism of ethanol extract from Hippocampus abdominalis on hyperuricemia in mice.Methods:The chemical components in the ethanol extract and the mouse serum were analyzed using high performance chemical isotope labeling(HP-CIL)technology.A mouse model of hyperuricemia was established by co-administration of potassium oxazinate and hypoxanthine.Serum levels of uric acid,creatinine,and uric acid nitrogen,as well as hepatic xanthine oxidase activity,were detected using commercial detection kits.HE staining was used to detect renal injury in mice.Western blot was used to detect protein expression levels.High throughput sequencing was used to analyze the changes in the gut microbiota in mice.Results:Chemical analysis identified 1 174 compounds in the hippocampal extract,among which peptides,amino acids,carboxylic acids,and fatty acids were the main ones.Eight compounds(tyrosyl-proline,isoleucyl-serine,threoninyl-alanine,tryptophyl-serine,prostaglandin D2,mataric acid,4-pyridoxic acid,and citric acid)were exclusively present in the serum of hippocampal extract-treated mice compared with normal and hyperuricemic mice and these compounds were also were found in the extract,suggesting their absorption into the blood circulation.Compared with the model mice,the extract significantly reduced serum uric acid levels.It also significantly reduced serum creatinine,uric acid nitrogen levels and alleviated renal injury,indicating renal protection.The ethanol extract inhibited hepatic xanthine oxidase and suppressed the renal expression of uric acid reabsorption transporters(URAT1 and GLUT9),while promoting the expression of uric acid excretion transporters(ABCG2,OAT1,and OAT3)in kidney tissue,indicating that H.abdominalis extract may regulate the level of blood uric acid in mice by affecting uric acid production,excretion and reabsorption.Furthermore,the extract significantly modulated the abundance of gut microbiota in hyperuricemic mice,restoring it to nearly normal levels.This demonstrates that the extract can regulate blood uric acid levels by remodeling intestinal microecology.Conclusion:H.abdominalis ethanol extract significantly ameliorates hyperuricemia in mice by regulating xanthine oxidase activity,uric acid transporter expression,and the gut microbiota.关键词
膨腹海马/高尿酸血症/尿酸排泄蛋白/尿酸重吸收蛋白/肠道菌群Key words
Hippocampus abdominalis/hyperuricemia/uric acid excretion proteins/uric acid reabsorption proteins/gut microbiota分类
医药卫生引用本文复制引用
张嘉媛,冯碧芸,李玲燕,杨欣慧,孔亚玲,王苏萍,李明,许光辉..膨腹海马乙醇提取物改善小鼠高尿酸血症的作用机制[J].食品科学,2026,47(5):162-173,12.基金项目
厦门市卫生健康高质量发展科技计划项目(2024GZL-CX43) （2024GZL-CX43）
厦门市自然科学基金项目(3502Z202373139) （3502Z202373139）
厦门市扶持中医药发展专项(XWZY-2025-0536 （）
XWZY-2025-0317) （）
福建省医学创新课题(2024CXB027) （2024CXB027）
