食品科学2026，Vol.47Issue(6)：23-40,18.DOI:10.7506/spkx1002-6630-20251013-059
基于多功能适配体磁性COF-CuO/Au纳米酶的双信号比色/荧光法检测海产品中副溶血性弧菌
Dual-Signal Colorimetric/Fluorescent Detection of Vibrio parahaemolyticus in Seafood Using a Multifunctional Aptamer-Conjugated Magnetic Covalent Organic Framework-CuO/Au Nanozyme
摘要
Abstract
In this study,a multifunctional aptamer-conjugated magnetic covalent organic framework(COF)-CuO/Au nanozyme(MCOF-CuO/Au@apt)was developed as a"three-in-one"platform for dual-signal colorimetric and fluorescent detection of Vibrio parahaemolyticus.The nanozyme integrated magnetic separation,peroxidase-like catalytic activity,and specific target recognition through an aptamer-based strategy.Upon binding to V.parahaemolyticus,the catalytic oxidation of tetra-aminophenylethylene(TPE-4A)by the nanozyme was selectively inhibited,resulting in distinct colorimetric and fluorescent signals that significantly enhanced the detection accuracy and reliability.The proposed method exhibited high sensitivity,with limits of detection(LOD)of 21 and 7 CFU/mL for the colorimetric and fluorescent assays,respectively.The performance of this method was validated using real seafood samples,including Penaeus vannamei,Mytilus coruscus,and Crassostrea gigas,which showed high recovery rates(101.11％–107.30％)and excellent reproducibility.The system also demonstrated strong specificity and accuracy under various conditions,confirming its robustness and practical applicability.Collectively,this innovative platform presents a promising solution for the rapid,versatile,and sensitive detection of V.parahaemolyticus in seafood,with considerable potential to advance food safety diagnosis and on-site monitoring.关键词
副溶血性弧菌/双信号检测/适配体纳米酶/磁性共价有机框架/海产品安全Key words
Vibrio parahaemolyticus/dual-signal detection/aptamer-based nanozyme/magnetic covalent organic framework/seafood safety分类
轻工纺织引用本文复制引用
孙迪,杨学文,王辉,林鸿勇,何晓霞,霍震霆,刘宇,余中节,姜维..基于多功能适配体磁性COF-CuO/Au纳米酶的双信号比色/荧光法检测海产品中副溶血性弧菌[J].食品科学,2026,47(6):23-40,18.基金项目
国家海洋设施养殖工程技术研究中心开放课题项目(NERC202410) （NERC202410）
国家自然科学基金青年科学基金项目(32402261 （）
32402034) （）
广东省水产品加工与安全重点实验室开放基金项目(GDPKLAPPS2409) （GDPKLAPPS2409）
大学生科技成果推广项目(2025XMPY020) （2025XMPY020）
