茶叶科学2026,Vol.46Issue(2):207-220,14.
'望海茶1号'配套扦插繁育技术和不定根转录特性分析
Cutting Propagation Technology and Transcription Characteristics Analysis of Adventitious Roots for'Wanghaicha 1'
摘要
Abstract
'Wanghaicha 1'is a newly bred green tea cultivar origining from the local population in Ninghai county.To establish an efficient breeding system,a comparative cutting experiment of long and short branch cuttings in lightweight non-woven seedling bags,and a short branch field mulching cutting experiment were set up at the Shengzhou base.The results show that the rooting rate of short branch cuttings of this cultivar was higher than that of the control'Longjing 43',with better growth and greater tolerance to high temperatures and drought.Moreover,the long branch cuttings can reach the nursery standard after 77 days.The method of short branch cuttings covered with black non-woven fabric film on the seedbed can effectively reduce the frequency of manual weeding.And through Illumina HiSeq platform,transcriptome sequencing of the root system of'Wanghaicha 1'was performed.Compared with the control'Longjing 43',107 differentially expressed genes related to plant hormones were screened.It is speculated that the synergistic regulation of these genes may be the molecular basis for the developed root system of this culitvar.This study preliminarily obtained 107 differentially expressed genes related to plant hormones in the adventitious roots of'Wanghaicha 1'.The established cutting mode of'early emergence of long branch from the nursery application of black plastic film to improve quality and efficiency'can shorten the seedling period by 15.4%and reduce weed control costs by 85.7%,providing theoretical basis and technical support for the industrial promotion of new cultivar.关键词
茶树新品种/育种/扦插技术/不定根/转录组Key words
new tea cultivar/breeding/cutting techniques/adventitious root/transcriptome分类
农业科技引用本文复制引用
姜燕华,何梦迪,胡桐,韩震,陈映男,吴立赟,韦康,王丽鸳..'望海茶1号'配套扦插繁育技术和不定根转录特性分析[J].茶叶科学,2026,46(2):207-220,14.基金项目
浙江省农业新品种选育重大专项-茶树(2021C02067-7-1) (2021C02067-7-1)
国家茶叶产业技术体系(CARS-19) (CARS-19)