现代食品科技2026，Vol.42Issue(3)：64-74,11.DOI:10.13982/j.mfst.1673-9078.2026.3.0031
转录因子PdTP1在指状青霉中调控α-松油醇合成及菌株生长的分子机制
Molecular Mechanism of the Transcription Factor PdTP1 in Regulating α-Terpineol Biosynthesis and Fungal Growth in Penicillium digitatum
摘要
Abstract
To enhance the regulatory network of the Zn2Cys6-type transcription factor,PdTP1,this study employed transcriptome sequencing technology to screen for genes related to the biotransformation of limonene into α-terpineol by Penicillium digitatum DSM 62840 and the regulation of fungal growth.Transcriptome sequencing was performed on the wild-type P.digitatum DSM 62840 and PdTP1-silenced strains to investigate the metabolic pathways and biological functions of key differentially expressed genes(DEGs).Reliability of the transcriptome sequencing results was verified through quantitative real-time PCR.A total of 1 221 DEGs,including 499 upregulated and 722 downregulated genes,were identified.GO and KEGG analyses indicated that these DEGs were significantly enriched in the metabolism and biosynthesis of various substances,including sugars,amino acids,and fatty acids.Among them,the expression of certain genes related to terpene metabolism or synthesis,fungal growth,and the stress response were significantly regulated by PdTP1,suggesting that these genes may play important roles in regulating the biotransformation of limonene to α-terpineol and the growth of P.digitatum.Reliability of the transcriptome sequencing results was confirmed by the quantitative real-time PCR results.In summary,this study refined the regulatory network of the transcription factor,PdTP1,by examining variations in gene transcription regulated by it,providing evidence for elucidating the molecular mechanisms by which PdTP1 regulates α-terpineol biosynthesis and fungal growth.关键词
转录因子PdTP1/α-松油醇/菌株生长/转录组/基因Key words
PdTP1/α-terpineol/fungal growth/transcriptome/genes引用本文复制引用
黄佳琳,张婷,王子轩,张佳月,王婧玥,安康,程慧如,王静文,张璐璐..转录因子PdTP1在指状青霉中调控α-松油醇合成及菌株生长的分子机制[J].现代食品科技,2026,42(3):64-74,11.基金项目
国家自然科学基金青年基金项目(32101868) （32101868）
中国博士后科学基金面上项目(2024M760795) （2024M760795）
河南省博士后启动经费资助项目(HN2025039) （HN2025039）
河南省科技研发计划联合基金(242103810080) （242103810080）
河南工业大学高层次人才基金项目(2021BS054) （2021BS054）
