肉类研究2026，Vol.40Issue(7)：73-80,8.DOI:10.7506/rlyj1001-8123-20250714-229
基于CRISPR/Cas技术的肉类掺假检测研究进展
Advances in Meat Adulteration Detection Based on CRISPR/Cas Technology
摘要
Abstract
In recent years,meat adulteration has become a prominent issue threatening food safety and touching on religion,economy,and more,making it a pressing social problem.While various detection methods exist for meat adulteration,traditional techniques fall short in meeting the demand for rapid and accurate on-site detection.Thus,developing rapid and accurate methods for on-site detection of meat adulteration is of great significance.The clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated proteins(Cas)system,composed of CRISPR and Cas,has the advantages of trans-cleavage activity and high specificity and has become an ultrasensitive nucleic acid detection tool.It shows significant potential in meat adulteration detection.This paper reviews the pros and cons of the existing methods for meat adulteration detection,and introduces the composition,classification,and mechanism of action of the CRISPR/Cas system,with a focus on the application of CRISPR/Cas-based technology in meat adulteration detection.It also discusses the advantages,challenges and future directions of this technology.关键词
成簇规则间隔短回文重复序列及其相关蛋白/核酸检测/肉类掺假Key words
clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated proteins/nucleic acid detection/meat adulteration分类
轻工纺织引用本文复制引用
郭润泽,张颖,杨瑞琴,孙启凡..基于CRISPR/Cas技术的肉类掺假检测研究进展[J].肉类研究,2026,40(7):73-80,8.基金项目
"十四五"国家重点研发计划重点专项(2022YFC3341002) （2022YFC3341002）
