食品工业科技2026，Vol.47Issue(9)：421-429,9.DOI:10.13386/j.issn1002-0306.2025050312
海带寡糖对果蝇抗氧化酶活性及抗衰老作用研究
Effects of Laminaria japonica Oligosaccharides on Antioxidant Enzyme Activities and Anti-aging in Drosophila melanogaster
摘要
Abstract
Objective:To evaluate the antioxidant capacity and anti-aging potential of Laminaria japonica oligosaccharides 2(LOs2)in Drosophila melanogaster.Method:Wild-type vestigial-wing D.melanogaster were reared on standard medium supplemented with LOs2 at low(0.25%,w/w),medium(0.50%),or high(1.00%)doses.The antioxidant and anti-aging effects of LOs2 were evaluated by determining the activity of superoxide dismutase(SOD),the content of malonic dialdehyde(MDA),the activity of catalase(CAT)in D.melanogaster,and the lifespan experiment of D.melanogaster.Result:Relative to the blank control,the 1.00%LOs2 group showed the highest T-AOC,increasing by 45.87%in females and 76.21%in males.MDA levels were markedly reduced(82.77%in females,61.24%in males)(P<0.05).SOD and CAT activities rose significantly at 1.00%LOs2 in both sexes(P<0.05).The 1.00%LOs2 group showed a 54.13%increase in female lifespan and a 72.00%increase in male lifespan compared to the blank control group.Consistently,qPCR revealed marked up-regulation of SOD1,SOD2,and CAT in the 1.00%group(P<0.05).Conclusion:These findings suggested that LOs2 attenuated the accumulation of excessive ROS and MDA by up-regulating the expression of SOD1,SOD2,and CAT genes,thereby delaying aging of D.melanogaster.LOs2 exhibited favorable antioxidant activity in vitro and in vivo,as well as potential anti-aging effects.关键词
海带寡糖/抗氧化/抗衰老/果蝇Key words
Laminaria japonica oligosaccharides/antioxidant/anti-aging/Drosophila melanogaster分类
轻工纺织引用本文复制引用
王蕾,邱佳怡,周知锦,陈甜怡,谢颖星,蔡旭正,汪财生,钱国英..海带寡糖对果蝇抗氧化酶活性及抗衰老作用研究[J].食品工业科技,2026,47(9):421-429,9.基金项目
宁波余姚市农社科类科技创新项目(2025JH03010017). （2025JH03010017）
