食品工业科技2026，Vol.47Issue(10)：322-334,13.DOI:10.13386/j.issn1002-0306.2025040259
不同烘干温度的黔产红托竹荪非靶向代谢组学研究
Non-targeted Metabolomics Study of Dictyophora rubrovalvata from Guizhou at Different Drying Temperatures
摘要
Abstract
As a rare and valuable edible fungus native to Guizhou Province,challenges in industrial upgrading due to the impact of drying temperatures on the retention of functional components during dry product processing were faced by Dictyophora rubrovalvata(DR).In this study,the investigation on air-source heat pump-dried DR samples at 40,45,50,and 55℃(labeled as DR40,DR45,DR50,and DR55,respectively)was conducted using liquid chromatography-mass spectrometry(LC-MS)-based non-targeted metabolomics.By using principal component analysis(PCA),orthogonal partial least squares discriminant analysis(OPLS-DA),KEGG pathway enrichment analysis,and differential abundance(DA)score analysis,dynamic metabolic changes and network responses under varying drying temperatures were systematically characterized.The detection of a total of 4470 metabolites was conducted,primarily distributed across 21 major classes(88.46%of total),including lipids,fatty acyls,and steroids.At 40～45℃,metabolic stability was high,with only 1071 differential metabolites(DR45 vs.DR40),in which a small disparity between upregulated(757)and downregulated(314)metabolites was shown.At 50℃,differential metabolites increased to 1219(DR50 vs.DR40),with significantly more upregulated(993)than downregulated(226)metabolites,indicating metabolic system activation to maintain homeostasis.At 50～55℃,differential metabolites sharply decreased to 577(DR55 vs.DR50),with downregulated metabolites(328)surpassing upregulated ones(249),so metabolic system damage beyond the temperature threshold was presented.From KEGG enrichment analysis,high temperatures(≥50℃)were shown to significantly affect lipid,flavonoid,and antioxidant-related pathways,so metabolic network reconstruction was caused.Based on the study,a temperature-sensitive threshold of approximately 50℃for DR drying was confirmed,with 40～45℃preserving original metabolic characteristics,thus offering a theoretical basis for optimizing industrial drying processes,recommending temperatures below 50℃to minimize functional component loss.关键词
红托竹荪/干燥温度/非靶向代谢组学/代谢通路/代谢物Key words
Dictyophora rubrovalvata/drying temperature/untargeted metabolomics/metabolic pathways/metabolites分类
农业科技引用本文复制引用
郭银萍,黄晓润,龚光禄,刘宏宇,黄万兵,卢颖颖,刘倾城,杨通静..不同烘干温度的黔产红托竹荪非靶向代谢组学研究[J].食品工业科技,2026,47(10):322-334,13.基金项目
贵州省科技计划项目(黔科合基础-ZK[2023]一般172) （黔科合基础-ZK[2023]一般172）
贵州省科技计划项目(黔科合成果[2024]一般132号) （黔科合成果[2024]一般132号）
贵州省2024年基层农技推广补助项目 （）
贵州省农业科学院青年科技基金项目(黔农科一般基金[2025]29). （黔农科一般基金[2025]29）
