食品与发酵工业2026，Vol.52Issue(8)：1-8,中插1-中插6,14.DOI:10.13995/j.cnki.11-1802/ts.044122
基于理化、酶活力和不挥发化合物解析不同等级高温大曲异质性
Analysis of heterogeneity of high-temperature Daqu at different grades based on physicochemical properties,enzyme activity,and non-volatile compounds
摘要
Abstract
The quality of high-temperature Daqu significantly impacts the yield and quality of Jiangxiangxing Baijiu.This study char-acterized the heterogeneity of high-temperature Daqu at different grades using physicochemical indicators,enzymatic activity analysis,and derivatization-GC-MS technology to detect non-volatile compounds.Results indicated that the premium-grade Daqu exhibited significantly higher acidity,protease activity,and amino acid nitrogen content than the normal-grade Daqu.Conversely,the normal grade Daqu had higher moisture,starch content,protein content,and saccharification enzyme activity.105 non-volatile compounds were identified by qual-itative and quantitative analysis.The total non-volatile compound content was highest in the premium-grade Daqu,primarily consisting of amino acids(19％-28％),sugars(25％-41％),and sugar alcohols(20％).Amino acid content increased with Daqu quality,while sugar content decreased.Seventeen key non-volatile compounds(variable importance in the projection＞1,|log2fold change|≥1,P＜0.05)were identified by partial least squares-discriminant analysis(PLS-DA).The premium-grade Daqu was enriched in 11 key com-pounds,including pyroglutamic acid and mannitol,whereas the normal-grade Daqu showed significant accumulation of ribitol,xylose,glucose,glutamic acid,and lactic acid.These results provide a theoretical basis for analyzing quality differences between high-temperature Daqu of different grades and offer insights for controlling and enhancing quality.关键词
高温大曲/不同等级/不挥发化合物/理化/酶活力/衍生化Key words
high-temperature Daqu/different grades/non-volatile compounds/physicochemical properties/enzymatic activity/chemical derivatization引用本文复制引用
张坤坤,范文来,徐岩,唐绍培,李婷婷,万心怡..基于理化、酶活力和不挥发化合物解析不同等级高温大曲异质性[J].食品与发酵工业,2026,52(8):1-8,中插1-中插6,14.基金项目
国家重点研发计划项目(2022YFD2101201) （2022YFD2101201）
