食品与发酵工业2026，Vol.52Issue(8)：96-102,7.DOI:10.13995/j.cnki.11-1802/ts.044856
蛹虫草活性多肽的制备及其神经保护作用探究
Preparation of bioactive peptides from Cordyceps militaris and investigation of their neuroprotective effects
摘要
Abstract
Cordyceps militaris is a high-quality functional food rich with various bioactive substances.This study aimed to extract pro-teins from C.militaris and prepare bioactive peptides through specific enzymatic hydrolysis,followed by separation,purification,and iden-tification.The neuroprotective effects of these bioactive peptides were investigated.Results showed that hydrolysates derived from C.milita-ris proteins through simulated gastrointestinal digestion exhibited significant neuroprotective activity.Ultrafiltration was used to fractionate the hydrolysates,and the hydrolysate fraction with＜3 kDa demonstrated the strongest neuroprotective effects.Further analysis identified 76 small peptides within this fraction as the main contributors to its bioactivity,which was closely associated with the high content of hy-drophobic amino acids and aromatic amino acids.Among these peptides,a specific peptide monomer,NWVPGD,was identified.NWVPGD effectively mitigated glutamate-induced damage in PC12 cells,exhibited significant free radical scavenging activity,and demon-strated the potential to activate the Keap1-Nrf2 antioxidant pathway.Its antioxidant properties likely played a critical role in its neuropro-tective effects.This study successfully identified and characterized a well-defined bioactive peptide from C.militaris,providing support for the development of C.militaris-derived peptides with neuroprotective functions.关键词
蛹虫草/多肽/模拟胃肠消化/抗氧化/神经保护Key words
Cordyceps militaris/peptide/simulated gastrointestinal digestion/antioxidant/neuroprotection引用本文复制引用
游晟宇,林岱,吴俊辉,李国凤,吴书建..蛹虫草活性多肽的制备及其神经保护作用探究[J].食品与发酵工业,2026,52(8):96-102,7.基金项目
国家自然科学基金青年科学基金项目(82504384) （82504384）
福建省自然科学基金项目面上项目(2025J01759) （2025J01759）
福建医科大学高层次人才引进项目(XRCZX2024029) （XRCZX2024029）
深圳市科技计划资助(JCYJ20230807142310022) （JCYJ20230807142310022）
