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贡柑浊汁的蛋白组学及起云蛋白特性分析

王锋 刘祯 马路凯 王琴 肖更生 刘东杰

食品与机械2026,Vol.42Issue(2):45-50,6.
食品与机械2026,Vol.42Issue(2):45-50,6.DOI:10.13652/j.spjx.1003.5788.2025.80363

贡柑浊汁的蛋白组学及起云蛋白特性分析

Proteomics of Tribute citrus turbid juice and characteristic analysis of cloudy proteins

王锋 1刘祯 1马路凯 1王琴 1肖更生 1刘东杰1

作者信息

  • 1. 仲恺农业工程学院轻工食品学院,广东 广州 510225||农业农村部岭南特色食品绿色加工与智能制造重点实验室,广东 广州 510225||广东省岭南特色食品科学与技术重点实验室,广东 广州 510225
  • 折叠

摘要

Abstract

[Objective]This study aims to investigate the characteristics of protein components and the types of cloudy proteins in Tribute citrus turbid juice.[Methods]Proteomics was used to analyze the proteomic features of the supernatant proteins and precipitated proteins in Tribute citrus turbid juice.[Results]There were significant differences in the electrophoresis bands of the supernatant proteins and precipitated proteins in Tribute citrus turbid juice.Compared with the supernatant proteins,precipitated proteins contained 2 897 differential proteins,including 2 487 up-regulated differential proteins and 410 down-regulated differential proteins.The up-regulated differential proteins in Tribute citrus turbid juice were mainly enriched in metabolic pathways including tyrosine metabolism,fatty acid degradation,and phagosome,mainly from cellular components such as chloroplasts,cytoplasm,and nucleus.Six types of cloudy proteins were screened from up-regulated differential proteins in Tribute citrus turbid juice,including one pathogenesis-related protein and five glycoproteins.[Conclusion]Proteomics can be used to explore the protein differences in Tribute citrus turbid juice.

关键词

贡柑/浊汁/蛋白组学/差异蛋白/起云蛋白

Key words

Tribute citrus/turbid juice/proteomics/differential protein/cloudy protein

引用本文复制引用

王锋,刘祯,马路凯,王琴,肖更生,刘东杰..贡柑浊汁的蛋白组学及起云蛋白特性分析[J].食品与机械,2026,42(2):45-50,6.

基金项目

国家自然科学基金(编号:U22A20543) (编号:U22A20543)

"十四五"广东省农业科技创新十大主攻方向"揭榜挂帅"项目(编号:2024KJ15) (编号:2024KJ15)

广东省重点建设学科科研能力提升项目(编号:2022ZDJS021,2024ZDJS006) (编号:2022ZDJS021,2024ZDJS006)

食品与机械

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