食用菌学报2026，Vol.33Issue(2)：52-65,14.DOI:10.16488/j.cnki.1005-9873.2026.02.005
木蹄层孔菌生物学特性及驯化
Biological Characteristics and Domestication of Fomes fomentarius
摘要
Abstract
Wild fruiting bodies of Fomes fomentarius were collected from Betula platyphylla in Saihanba Mechanised Tree Farm,Weichang County,Chengde City,Hebei Province.After tissue isolation,a strain was identified as F.fomentarius,and then the strain was studied for biological characteristics through single-factor experiments and orthogonal experiments.Finally,the strain was domesticated.The results showed that the optimal carbon source,nitrogen source,temperature,and initial pH in single-factor experiments were fructose,yeast extract powder,28 ℃,and pH7,respectively.The optimal conditions determined by the orthogonal experiments were fructose,yeast extract powder,28 ℃,and pH6.After inoculation,the mycelia of the isolated F.fomentarius strain fully colonized the cultivation bags within 50-55 d,and hemispherical primordia formed after an additional 40-45 d.Harvesting was possible after a further 25-35 d of cultivation,with the fruiting bodies measuring 4.5-6.4 cm in diameter and having a base thickness of 2.5-3.0 mm.These findings provided a reference for subsequent development and utilization of F.fomentarius.关键词
药用菌/木蹄层孔菌/单因素/正交实验Key words
Medicinal fungi/Fomes fomentarius/single factor/orthogonal experiment引用本文复制引用
姚英杰,康霞,赵怡,王立安,李守勉,李国杰..木蹄层孔菌生物学特性及驯化[J].食用菌学报,2026,33(2):52-65,14.基金项目
河北省食用菌产业创新团队珍稀食用菌岗位(HBCT2023090202) （HBCT2023090202）
河北省自然科学基金项目(C2025204257) （C2025204257）
河北农业大学创新创业训练计划项目(2024263) （2024263）
河北省科技计划项目重点研发计划(21326315D) （21326315D）
河北省县域生物多样性调查评估项目(2011600012) （2011600012）
