中国食品添加剂2026，Vol.37Issue(4)：53-59,7.DOI:10.19804/j.issn1006-2513.2026.4.007
高氧-褪黑素协同抑制PLA1β2-PG酶延缓枸杞采后劣变机制
Mechanism of synergistic inhibition of PLA1β2-PG enzyme by high oxygen and melatonin to delay post-harvest deterioration of goji berries
许强 1雷倩2
作者信息
- 1. 南京建康高级技工学校,南京 211156
- 2. 安徽大学物质科学与信息技术研究院,合肥 230601
- 折叠
摘要
Abstract
A synergistic treatment strategy of high oxygen(80%O2)and melatonin(1.0 mmol/L)was proposed to address the softening and browning issues caused by disrupted cell wall metabolism in fresh goji berries(Lycium barbarum L.)after harvesting.Its regulatory effects and the underlying molecular mechanisms on storage quality of fresh goji berries were systematically analyzed.Fruit firmness and browning index were measured,and PLA1β2 gene expression,polygalacturonase(PG)activity and dynamic changes in cellwall components were analyzed.It was found that synergistic treatment significantly inhibited PLA1β2 gene transcription(downregulated by 55.0%)and reduced PG enzyme activity(decreased by 50.4%),the degradation of covalently bound pectin(CSP)was delayed,and the shelf life of fresh goji berries was extended from 15 days in the control group to 25 days under the combined treatment.Pearson correlation analysis showed a highly significant positive correlation between browning index and PLA1β2 expression level(r=0.89,P＜0.01),indicating their core roles in quality deterioration of fresh goji berries.This study provided a theoretical basis for the development of green preservation technology for fresh goji berries.关键词
鲜食枸杞/高氧处理/褪黑素/PLA1β2基因/PG酶活性/细胞壁降解Key words
fresh goji berry/high oxygen treatment/melatonin/PLA1β2 gene/PG enzyme activity/cell wall degradation分类
轻工纺织引用本文复制引用
许强,雷倩..高氧-褪黑素协同抑制PLA1β2-PG酶延缓枸杞采后劣变机制[J].中国食品添加剂,2026,37(4):53-59,7.
