乳业科学与技术2026，Vol.49Issue(2)：19-23,5.DOI:10.7506/rykxyjs1671-5187-20251005-065
高效液相色谱法检测液态乳中5-羟甲基糠醛与糠醛含量
Determination of 5-Hydroxymethylfurfural and Furfural Contents in Liquid Milk by High-Performance Liquid Chromatography
赵姝丽 1徐红 1唐振鑫 1冀何连 1张雪娇 1王忱 1高万东 1张彩霞 1吴晓莉1
作者信息
- 1. 内蒙古伊利实业集团股份有限公司,国家市场监督管理总局重点实验室(牛羊乳肉制品风险防控与关键技术),内蒙古 呼和浩特 010110
- 折叠
摘要
Abstract
A high-performance liquid chromatography method was developed to determine the contents of 5-hydroxymethylfurfural and furfural in liquid milk.The samples were extracted with oxalic acid solution in a boiling water bath,then the protein and fat were precipitated by adding trichloroacetic acid solution.The supernatant was separated on an XDB-C18 column(4.6 mm×250 mm,5 μm)using 10%acetonitrile solution as the mobile phase.The analytes were detection with an ultraviolet detector and quantified by the external standard method.The experimental results showed that the correlation coefficient of 5-hydroxymethylfurfural was 0.999 8,with a spiked recovery ranging from 84.6%to 94.3%,a limit of detection(LOD)of 0.005 mg/kg,and a coefficient of variation(CV)of 0.15%to 0.36%.For furfural,the correlation coefficient was 0.999 4,with a spiked recovery rate ranging from 73.7%to 92.0%,a LOD of 0.005 mg/kg,and a CV of 0.95%to 1.54%.This method is characterized by simple operation,low cost,and excellent separation performance,and it is suitable for the quantitative analysis of 5-hydroxymethylfurfural and furfural in liquid milk,providing technical support for the quality control and process optimization of dairy products.关键词
糠醛/5-羟甲基糠醛/高效液相色谱/液态乳Key words
furfural/5-hydroxymethylfurfural/high-performance liquid chromatography/liquid milk分类
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赵姝丽,徐红,唐振鑫,冀何连,张雪娇,王忱,高万东,张彩霞,吴晓莉..高效液相色谱法检测液态乳中5-羟甲基糠醛与糠醛含量[J].乳业科学与技术,2026,49(2):19-23,5.
