乳业科学与技术2026，Vol.49Issue(2)：24-29,6.DOI:10.7506/rykxyjs1671-5187-20251118-080
基质辅助激光解吸电离飞行时间质谱鉴定沙门氏菌国标方法优化
Optimization of the National Standard Method for Salmonella Identification Using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry
摘要
Abstract
The rapid identification of Salmonella,a significant foodborne pathogen,is of significant importance.To establish a new method for identifying Salmonella based on matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS),this study optimized culture medium type,incubation time,bacterial lysis methods,and instrumental parameters.The results showed that compared to the national standard method GB/T 33682-2025 General microorganism identification method with matrix-assisted laser desorption/ionization time of flight mass spectrometry,the recovery rate increased from 54.7%to 84.7%after replacing the culture medium with nutrient agar.Increasing the incubation time from 24 to 48 h increased the average identification score by 7.0%,from 2.203 to 2.355.Optimizing lysis methods elevated the average identification score by 19.0%,from 1.898 to 2.259.When the instrumental parameters were adjusted to 50%laser energy and a laser wavelength of 370 nm,the average identification score increased to 2.211.Compared to its original version specified in GB/T 33682-2025,the established MALDI-TOF/TOF MS method exhibited improved accuracy and reliability in Salmonella identification.关键词
沙门氏菌/基质辅助激光解吸电离飞行时间质谱/方法优化/快速鉴定Key words
Salmonella/matrix-assisted laser desorption ionization time-of-flight mass spectrometry/method optimization/rapid identification分类
轻工纺织引用本文复制引用
张捷,张雅伦,张涛,陈晨,张子仑,周巍..基质辅助激光解吸电离飞行时间质谱鉴定沙门氏菌国标方法优化[J].乳业科学与技术,2026,49(2):24-29,6.基金项目
国家市场监督管理总局科技计划项目(2022MK013) （2022MK013）
